LEUKEMIA-ASSOCIATED CHANGES IDENTIFIED BY QUANTITATIVE FLOW-CYTOMETRY .1. CD10 EXPRESSION
LEUKEMIA-ASSOCIATED CHANGES IDENTIFIED BY QUANTITATIVE FLOW-CYTOMETRY .1. CD10 EXPRESSION
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DOI：
10.1002/cyto.990180404
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发表时间：
1994-12-15
期刊：
影响因子：
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通讯作者：
PORWITMACDONALD, A
中科院分区：
文献类型：
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作者：
LAVABREBERTRAND, T;JANOSSY, G;PORWITMACDONALD, A
我们比较了B系急性淋巴细胞白血病（ALL）和正常胎儿骨髓中CD 10抗原的表达。使用量子珠的定量间接免疫荧光（QIFI）和直接免疫荧光（IF）试验将中值荧光强度（MFI）值转换为每个细胞表达的抗原分子数（AgE），胎儿骨髓和肝脏中的类血小板前体表达3-12.5 x 10（3）CD 10分子/细胞，上限为5 x 10（4）/细胞（MaxAgE），在不同的急性B系ALL病例中，CD 10 AgE的中位数是可变的，范围从检测不到到非常高的值（> 1.8 x 10（5））。在72例接受QIFI检测的病例中，有24例（33%）的CD 10 AgE中位数高于正常样本中的最高值（> 5 x 10（4）/细胞）。另外23.6%的ALL患者的CD 10 AgE中位数高于正常值。其次，在常规的B系白血病多参数分析中，采用CD 10/II类/CD 34三色IF试验或CD 10/TdT双色IF试验对78例患者进行了CD 10抗原定量，43.6%的ALL患者证实了异常过度表达，CD 10（亮）显示提示ALL诊断，即使在可用于研究的细胞很少时，例如，在早期复发和以再生障碍性贫血为特征的急性淋巴细胞白血病中，CD 10表达水平在复发时保持不变，此外，不同的CD 10水平与各种染色体改变有关：高CD 10水平超二倍体的CD 10水平（> 3 x 10（4）/细胞）、t（1;19）的低CD 10水平（1.8-4 x 10（3）/细胞）和t（4;11）易位的不可检测水平（< 1.2 x 10（3）/细胞）。这些结果表明，虽然所有这些疾病都是前体B细胞谱的一部分，但CD 10的变化与疾病相关的改变有关，而不是真正反映正常B细胞前体亚型的特征，因此，定量CD 10评估应成为ALL常规流式细胞术评估的一部分，并且在化疗后的再生阶段需要进一步仔细研究。在强骨髓再生期间单独的高CD 10表达可能不是白血病特异性的，并且上述多参数分析和基因重排的平行研究对于建立这些测定在再生骨髓中检测微小疾病的相对值是必要的，（C）1994 Wiley-Liss，Inc.
We have compared CD10 antigen expression in normal fetal bone marrow with that of B-lineage acute lymphoblastic leukemia (ALL), Both quantitative indirect immunofluoresence (QIFI) and direct immunofluorescence (IF) tests with Quantum beads were used to convert median fluorescence intensity (MFI) values into numbers of antigen molecules expressed per cell (AgE), Lymphoid precursors in the fetal marrow and liver expressed 3-12.5 x 10(3) CD10 molecules/cell with an upper limit of 5 x 10(4)/cell (MaxAgE), The median CD10 AgE in the different cases of acute B-lineage ALL were variable and ranged from undetectable to very high values (> 1.8 x 10(5)). In 24 of the 72 cases (33%) tested with QIFI the median CD10 AgE was above the highest values seen in normal samples (> 5 x 10(4)/cell). An additional 23.6% of cases had higher median values than the normal median CD10 AgE, Next, CD10 antigen was quantitated in 78 cases during the routine multiparameter analysis of B-lineage leukemia using CD10/class II/CD34 3-color IF test or CD1O/TdT 2-color IF test, The aberrant overexpression was confirmed in 43.6% of ALL cases, The CD10(bright) display suggested ALL diagnosis even when few cells were available for study, e.g., in early relapse and in ALL masquerading as aplastic anemia, The levels of CD10 expression were maintained in relapse, In addition, different CD10 levels were associated with the various chromosomal alterations: high CD10 levels (> 3 x 10(4)/cell) with hyperdiploidy, low CD10 levels (1.8-4 x 10(3)/cell) with the t(1;19), and undetectable levels (< 1.2 x 10(3)/cell) with the t(4;11) translocations. These findings show that while all these diseases are part of the precursor B-cell spectrum, the CD10 changes are linked to disease-associated alterations instead of truly reflecting the features of subtypes of normal B-cell precursors, The quantitative CD10 assessment should therefore be part of the routine flow cytometric assessment in ALL, and further careful studies are warranted during the regeneration phase following chemotherapy. High CD10 expression alone during strong bone marrow regeneration may not be leukemia specific and both the multiparameter analysis described above and parallel studies for gene rearrangements will be necessary to establish the relative values of these assays in detecting minimal disease amidst regenerating marrow, (C) 1994 Wiley-Liss, Inc.