AUTORADIOGRAPHIC ANALYSIS OF THE DISTRIBUTION OF VASOACTIVE-INTESTINAL-PEPTIDE BINDING-SITES IN THE VERTEBRATE CENTRAL-NERVOUS-SYSTEM - A PHYLOGENETIC STUDY
AUTORADIOGRAPHIC ANALYSIS OF THE DISTRIBUTION OF VASOACTIVE-INTESTINAL-PEPTIDE BINDING-SITES IN THE VERTEBRATE CENTRAL-NERVOUS-SYSTEM - A PHYLOGENETIC STUDY
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DOI：
10.1016/0006-8993(90)91687-c
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发表时间：
1990-06-18
期刊：
影响因子：
2.9
通讯作者：
PALACIOS, JM
中科院分区：
文献类型：
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作者：
DIETL, MM;HOF, PR;PALACIOS, JM
采用体外放射自显影技术，对几种脊椎动物脑内血管活性肠肽(VIP)结合部位的分布进行了研究。这项研究包括鱼、青蛙、蛇、鸽子、大鼠、小鼠、豚鼠、猫和猴子的脑。在整个研究过程中，使用了一种完全表征的、单碘形式的血管活性肠肽(M-125I-VIP)，它在中枢神经系统(CNS)中保持了天然多肽的生物活性。在低等脊椎动物中，鱼的脑中没有发现明显的特异性结合，而在蛙和蛇的脑中，观察到了VIP的特异性结合部位，主要是在端脑。在鸽脑中，高密度的VIP结合部位位于纹状体上、新纹状体、弓状回、海马区、背外侧皮质区和视顶盖。外纹状体和古纹状体的特异性结合密度较低。在哺乳动物中，啮齿类动物大脑中VIP结合位点的浓度最高。在大鼠、小鼠和豚鼠脑内，嗅球、大脑皮层外层、齿状回、丘脑中线核、膝状核、下丘脑部分核团、上丘和蓝斑均有高密度分布。杏仁核、尾壳核、隔核、伏隔核、海马CA1~CA3区和中央灰质有中等密度。这些物种的小脑表现出高密度的VIP结合位点，其定位存在物种间的差异。然而，在啮齿动物的小脑中，这种非特异性结合增加。猫和猴中枢神经系统的VIP结合部位密度较低。在这两个物种中，非特异性结合显著高于低等哺乳动物的大脑。在猫和猴的脑中，与低等哺乳动物一样，在新皮质、齿状回、丘脑核团以及包括黑质和蓝斑在内的一些中脑结构中密度最高。在所有研究的物种中，白质从未被M-125I-VIP标记过。这项研究表明，VIP结合位点在脊椎动物中枢神经系统的进化中出现得相对较早。在鸽子和啮齿类动物的脑中观察到了最重要的VIP结合位点的密度，而猫和猴子的非特异性结合位点的密度显著增加。有趣的是，放射自显影显示的VIP结合位点的分布在进化上是相当保守的，表明VIP受体与参与特定感觉输入处理的大脑区域存在关联，尽管不是排他性的。
The distribution of vasoactive intestinal peptide (VIP) binding sites in the brain of several vertebrate species was examined by in vitro autoradiography on slide-mounted sections. This study included fish, frog, snake, pigeon, rat, mouse, guinea pig, cat and monkey brain. A fully characterized, monoiodinated form of vasoactive intestinal peptide (M-125I-VIP), which maintains the biological activity of the native peptide in the central nervous system (CNS), was used throughout the study. Among the lower vertebrate species, no significant specific binding was found in the fish brain, whereas in the frog and snake brain, specific VIP binding sites were observed, mainly in the telencephalon. In the pigeon brain, high densities of VIP binding sites were localized in the hyperstriatum, neostriatum, archistriatum, hippocampal area, dorsolateral cortical area and in the optic tectum. Ectostriatum and paleostriatum augmentatum displayed lower densities of specific binding. In mammals, the highest concentrations of VIP binding sites were observed in the rodent brain. In the rat, mouse and guinea pig brain, high densities were detected in the olfactory bulb, external layers of the cerebral cortex, dentate gyrus, midline thalamic nuclei, geniculate nuclei, some hypothalamic nuclei, superior colliculus and locus coeruleus. Intermediate densities were found in amygdala, caudate-putamen, septum and nucleus accumbens, CA1-CA3 fields of the hippocampus and central gray. The cerebellum of these species presented high densities of VIP binding sites, with species to species differences in their localization. The non-specific binding was, however, increased in the rodent cerebellum. Lower densities of VIP binding sites were observed in the cat and monkey CNS. In these two species, the non-specific binding was considerably higher than in the lower mammals brain. In the cat and monkey brain, as in the lower mammals, the highest densities were revealed in the neocortex, dentate gyrus, thalamic nuclei and some midbrain structures including substantia nigra and locus coeruleus. In all the species studied, the white matter was never labeled with M-125I-VIP. This study suggests that VIP binding sites appear relatively early in the evolution of the vertebrate CNS. The most important densities of specific VIP binding sites are observed in the pigeon and rodent brain, whereas the cat and monkey present a marked increase in non-specific binding. It is interesting to note that the distribution of VIP binding sites as revealed by autoradiography is quite conservative in terms of evolution and indicates an association, although non-exclusive, of VIP receptors with brain regions involved in the processing of specific sensory inputs.