Converging GABA‐ and glutamate‐immunoreactive axons make synaptic contact with identified hypothalamic neurosecretory neurons
Converging GABA‐ and glutamate‐immunoreactive axons make synaptic contact with identified hypothalamic neurosecretory neurons
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会聚的 GABA 和谷氨酸免疫反应性轴突与已识别的下丘脑神经分泌神经元形成突触接触
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. N. van den Pol
中科院分区：
文献类型：
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作者：
C. Decavel;A. N. van den Pol
为了研究大鼠下丘脑神经分泌神经元与突触接触轴突的神经化学特性，我们结合了标记分子的逆行轴突运输和氨基酸神经递质的着床后免疫金染色。静脉注射辣根过氧化物酶后，正中隆起或神经垂体中具有轴突的神经分泌神经元将过氧化物酶逆行运送回起源的细胞体。用谷氨酸或GABA抗血清对室旁核和弓状核的连续切片进行免疫染色。过氧化物酶标记的神经元和它们的树突接受来自对GABA或谷氨酸免疫反应的胶体金标记的轴突的突触接触。对GABA具有高度免疫反应性的轴突和对谷氨酸具有免疫反应性但对GABA没有免疫反应性的其他轴突始终与相同的过氧化物酶标记的细胞进行会聚突触接触。来自弓状核的一些过氧化物酶标记的神经元（其与GABA和谷氨酸轴突突触后）本身被鉴定为GABA免疫反应性。连续切片显示，多个突触前轴突免疫反应的谷氨酸或GABA与单个神经元反复接触。这些结果表明，在控制垂体前叶和后叶激素的神经元上，主要的抑制性和兴奋性氨基酸递质广泛会聚。
To study the neurochemical identity of axons in synaptic contact with identified hypothalamic neurosecretory neurons in rats, we combined retrograde axonal transport of a marker molecule with postembedding immunogold staining for ammo acid neurotransmitters. After intravenous injections of horseradish peroxidase, neurosecretory neurons with axons in the median eminence or neurohypophysis transported the peroxidase retrogradely back to the cell body of origin. Serial ultrathin sections from the paraventricular and arcuate nuclei were immunostained with glutamate or GABA antisera. Peroxidase‐labeled neurons and their dendrites received synaptic contact from colloidal gold‐labeled axons immunoreactive for GABA or for glutamate. Axons which were highly immunoreactive for GABA and other axons immunoreactive for glutamate but not for GABA consistently made converging synaptic contact with the same peroxidase‐labeled cell. Some of the peroxidase‐labeled neurons from the arcuate nucleus which were postsynaptic to both GABA and glutamate axons were themselves identified as being GABA immunoreactive. Serial ultrathin sections revealed that multiple presynaptic axons immunoreactive for glutamate or GABA made repeated contacts with single neurons. These results suggest a widespread convergence of the major inhibitory and excitatory amino acid transmitter on the neurons which control both the anterior and posterior pituitary hormones.
兴奋性氨基酸拮抗剂抑制豚鼠下丘脑室旁核的突触反应。
影响因子：
2.5
作者：
Wuarin,JP;Dudek,FE
通讯作者：
Dudek,FE
胶体金神经元成像。
影响因子：
2
作者：
vandenPol,AN
通讯作者：
vandenPol,AN