Lymphocytes are not required for the rapid onset of coronary heart disease in scavenger receptor class B type I/apolipoprotein E double knockout mice.
Lymphocytes are not required for the rapid onset of coronary heart disease in scavenger receptor class B type I/apolipoprotein E double knockout mice.
复制标题
在清道夫受体B类I型/载脂蛋白E双敲除小鼠中，冠心病的快速发作不需要淋巴细胞。
DOI：
10.1161/01.atv.0000158310.64498.ac
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Krieger,Monty
中科院分区：
文献类型：
--
作者：
Karackattu,SharonL;Picard,MichaelH;Krieger,Monty
Objective—Scavenger receptor class B type I (SR-BI)/apolipoprotein E (apoE) double knockout (dKO) mice exhibit many features of human coronary heart disease (CHD), including occlusive coronary atherosclerosis, cardiac hypertrophy, myocardial infarctions, and premature death. Here we determined the influence of B and T lymphocytes, which can contribute to atherosclerosis, ischemia–reperfusion injury, and cardiomyocyte death, on pathology in dKO mice.Method and Results—The lymphocyte-deficient SR-BI/apoE/recombination activating gene 2 (RAG2) triple knockout mice and corresponding dKO controls generated for this study exhibited essentially identical lipid-rich coronary occlusions, myocardial infarctions, cardiac dysfunction, and premature death (average lifespans 41.6±0.6 and 42.0±0.5 days, respectively).Conclusions—B and T lymphocytes and associated immunoglobulin-mediated inflammation are not essential for the development and progression of CHD in dKO mice. Strikingly, the dKO mice bred for this study (mixed C57BL/6×SV129×BALB/c background; strain 2) compared with the previously described dKO mice (75:25 C57BL/6:SV129 background; strain 1) had a shorter mean lifespan and steeper survival curve, characteristics especially attractive for studying the effects of environmental, pharmacological, and genetic manipulations on cardiac pathophysiology.
