IL-10 signalling blockade at the time of immunization inhibits Human papillomavirus 16 E7 transformed TC-1 tumour cells growth in mice
IL-10 signalling blockade at the time of immunization inhibits Human papillomavirus 16 E7 transformed TC-1 tumour cells growth in mice
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免疫时阻断 IL-10 信号传导可抑制小鼠体内人乳头瘤病毒 16 E7 转化的 TC-1 肿瘤细胞的生长。
DOI：
10.1016/j.cellimm.2014.06.002
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发表时间：
2014-07-01
影响因子：
4.3
通讯作者：
Wang, Yuejian
中科院分区：
文献类型：
--
作者：
Chen, Shu;Wang, Xiongfei;Wang, Yuejian
免疫时腹腔注射抗IL-10受体抗体阻断IL-10信号通路，增强疫苗诱导的CD8+ T细胞反应，促进细菌、寄生虫和病毒的控制。我们现在表明，在免疫时阻断IL-10信号传导可增强疫苗诱导的抗原特异性CD8+ T细胞对显性和亚显性CTL表位的反应。免疫时皮下注射抗il -10受体抗体也能增强CD8+ T细胞应答。此外，在人乳头瘤病毒16e7肽/LPS免疫时，IL-10信号阻断可阻止hpv16e7转化的TC-1肿瘤在小鼠中的生长。在抗il - 10r抗体和单磷酰脂质A存在的情况下免疫，产生抗原特异性CD8+ T细胞反应，类似于LPS免疫。我们的研究结果表明，免疫和IL-10信号阻断可能为开发治疗性癌症疫苗提供新的途径。(C) 2014爱思唯尔公司版权所有。
IL-10 signalling blockade by intra-peritoneal injection of anti-IL-10 receptor antibodies at the time of immunization enhances vaccine induced CD8+ T cell responses and promotes bacteria, parasitic and viral control. We now show that blockade of IL-10 signalling at the time of immunization enhances vaccine induced antigen specific CD8+ T cell responses to both dominant and subdominant CTL epitopes. Injection of anti-IL-10 receptor antibodies subcutaneous at the time of immunization also enhances CD8+ T cell responses. Furthermore, IL-10 signalling blockade at the time of a Human papillomavirus 16 E7 peptide/LPS immunization, prevents HPV16 E7 transformed TC-1 tumour growth in mice. Immunization in the presence of anti-IL-10R antibodies and Monophosphoryl lipid A, generates antigen specific CD8+ T cell responses similar to immunization with LPS. Our results suggest that immunization and IL-10 signalling blockade may provide a novel way for the development of therapeutic vaccines against cancer. (C) 2014 Elsevier Inc. All rights reserved.