Modelling directional guidance and motility regulation in cell migration
Modelling directional guidance and motility regulation in cell migration
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DOI：
10.1007/s11538-005-9028-x
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Hughes, BD
中科院分区：
文献类型：
--
作者：
Cai, AQ;Landman, KA;Hughes, BD
虽然细胞迁移是发育中的一个重要过程，但细胞如何到达其最终目的地尚不清楚。已知分泌的分子具有迁移作用，但仍不清楚这些分子是作为定向引导线索还是作为运动调节剂。在新的医学疗法中使用信号分子是有潜力的，因此确定这些分子发挥的确切作用是很重要的。本文着重于区分信号分子产生的抑制和排斥作用，基于最近的实验研究Slit，一种分泌蛋白，对神经元从大脑迁移的影响。Slit的主要作用，无论是神经元的抑制剂还是排斥剂，都存在争议。我们提出了人口水平的连续模型，并重铸这些方面的过渡概率管理个别细胞。在这个框架内，考虑各种细胞传感策略。将该模型应用于神经元迁移实验。为了解决狭缝的特殊作用，模拟的模型特征不同的细胞传感策略进行比较，在人口和个人的细胞水平，提供两个互补的角度对系统。讨论了从延时成像中推断细胞迁移规律的困难和局限性。
Although cell migration is an essential process in development, how cells reach their final destination is not well understood. Secreted molecules are known to have a migratory effect, but it remains unclear whether such molecules act as directional guidance cues or as motility regulators. There is potential to use signalling molecules in new medical therapies, so it is important to identify the exact role these molecules play. This paper focuses on distinguishing between inhibitory and repulsive effects produced by signalling molecules, based on recent experiments examining the effect of Slit, a secreted protein, on the migration of neurons from the brain. The primary role of Slit, whether it is ail inhibitor or repellent of neurons, is in dispute. We present population-level continuum models and recast these in terms of transition probabilities governing individual cells. Various cell-sensing strategies are considered within this framework. The models are applied to the neuronal migration experiments. To resolve the particular role of Slit, simulations of the models characterising different cell-sensing strategies are compared at the population and individual cell level, providing two complementary perspectives on the system. Difficulties and limitations in deducing cell migration rules from time-lapse imaging are discussed.