An in vivo randomized study of human skin moisturization by a new confocal Raman fiber-optic microprobe: Assessment of a glycerol-based hydration cream
An in vivo randomized study of human skin moisturization by a new confocal Raman fiber-optic microprobe: Assessment of a glycerol-based hydration cream
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DOI：
10.1159/000093116
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发表时间：
2006-01-01
影响因子：
2.7
通讯作者：
Hadjur, C.
中科院分区：
文献类型：
--
作者：
Chrit, L.;Bastien, P.;Hadjur, C.
背景资料：在最近的一项研究中，我们证明了新的共焦拉曼微探针的能力，在体内条件下，调查分子和结构的人体皮肤组成。实验在不同的解剖部位，不同的层次，并与志愿者之间的比较。我们还进行了可行性测试，使用这种探头，以确定皮肤内的水含量的深度剖面。目的：在目前的调查中，我们采用共焦拉曼光学微探针，严格客观化后的保湿增强剂的应用所产生的水合能力。方法：对26名健康志愿者进行了体内实验，并在随机应用保湿剂后对前臂掌侧的六个区域进行了测量。通过计算水/蛋白质条带比率来评估响应，该比率确定皮肤中的水含量。结果：用拉曼微探针收集的数据显示对照和治疗皮肤的基线值之间的显著变化。对这些数据进行的统计分析显示，在应用甘油基霜剂后皮肤水分增加，甘油基霜剂是最广泛使用的保湿剂。结论：我们的研究结果清楚地表明了这种共焦拉曼微探针在体内条件下筛选水合剂或分子的潜力。在化妆品领域，这种有前途的和适用的技术无疑将提供新的机会，水化皮肤测试评价。版权所有（c）2006 S. Karger AG，巴塞尔。
Background: In a recent study, we demonstrated the ability of the new confocal Raman microprobe to investigate molecular and structural human skin composition under in vivo conditions. Experiments were performed at different anatomical sites, different layers, and with intervolunteer comparison. We also carried out feasibility tests using this probe to determine depth profiles of water content within the skin. Objective: In the present investigation we employed this confocal Raman optical microprobe to rigorously objectify the resulting hydration capacities after application of a moisturizing enhancer. Method: The in vivo experiments were performed on 26 healthy volunteers and measurements were undertaken on six areas of the volar forearm after a randomized application of hydrating agents. Responses were evaluated by calculating the water/protein band ratio, which determines the water content in the skin. Results: Data collected with the Raman microprobe showed significant changes between baseline values of control and treated skins. Statistical analysis performed on these data revealed an increase in skin moisture after application of a glycerol-based cream, which is the most widely used hydrating agent. Conclusion: Our results demonstrate clearly the potentials of this confocal Raman microprobe in the screening of hydrating agents or molecules under in vivo conditions. In the cosmetics field, this promising and suitable technique will undoubtedly offer new opportunities of hydration skin test evaluation. Copyright (c) 2006 S. Karger AG, Basel.