Hyaluronan inhibits bone resorption by suppressing prostaglandin E synthesis in osteoblasts treated with interleukin-1
Hyaluronan inhibits bone resorption by suppressing prostaglandin E synthesis in osteoblasts treated with interleukin-1
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DOI：
10.1016/j.bbrc.2009.01.146
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发表时间：
2009-04-03
影响因子：
3.1
通讯作者：
Inada, Masaki
中科院分区：
文献类型：
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作者：
Hirata, Michiko;Kobayashi, Megumi;Inada, Masaki
透明质酸（HA）是一种大型糖胺聚糖，是多种组织中细胞外基质的组成部分。透明质酸对软骨的基质组装和液体黏度至关重要，但其在骨中的作用尚不清楚。炎症相关的骨吸收与白细胞介素-1 （IL-1）等细胞因子诱导成骨细胞合成前列腺素E （PGE）密切相关。在小鼠颅骨培养中，透明质酸抑制IL-1诱导的破骨细胞骨吸收和PGE的产生。在小鼠成骨细胞中，HA抑制il -1诱导的环氧化酶(COX)-2和膜结合PGE合成酶(mPGES)-1 mrna的表达和PGE(2)的产生。基质金属蛋白酶（MMPs），包括MMP-2和MMP-13，是由成骨细胞响应IL-1产生的，并明显受到HA的抑制。在成骨细胞中，在ILICiferase实验中，HA抑制NF κ b依赖性转录。因此，HA作用于成骨细胞抑制PGE2和MMPs的产生，并抑制骨吸收，提示HA在病理性骨质流失伴炎症中的重要作用。(C) 2009爱思唯尔公司版权所有。
Hyaluronan (HA), a large glycosaminoglycan, is a component of the extra-cellular matrix in various tissues. HA is essential for matrix assembly and fluid viscosity in cartilage, but the roles of HA in bone are unclear. Bone resorption associated with inflammation is closely related to prostaglandin E (PGE) synthesis by osteoblasts induced by cytokines such as interleukin-1 (IL-1). In mouse calvarial Cultures, HA inhibited osteoclastic bone resorption and PGE production induced by IL-1. In mouse osteoblasts, HA suppressed IL-1-induced expression of cyclooxygenase(COX)-2 and membrane-bound PGE synthase (mPGES)-1 mRNAs, and PGE(2) production. Matrix metalloproteinases (MMPs), including MMP-2 and MMP-13, were produced by osteoblasts in response to IL-1, and were clearly suppressed by HA. In osteoblasts, HA suppressed the NF kappa B-dependent transcription in a ILICiferase assay. Therefore, HA acts on osteoblasts to Suppress the production Of PGE2 and MMPs, and inhibits bone resorption, suggesting critical roles of HA in pathological bone loss with inflammation. (C) 2009 Elsevier Inc. All rights reserved.