Investigation of phage and molasses interactions for the biocontrol of E. coli O157:H7
Investigation of phage and molasses interactions for the biocontrol of E. coli O157:H7
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DOI：
10.1139/cjm-2021-0211
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发表时间：
2022-01-01
影响因子：
2.8
通讯作者：
Boyaci, Ismail Hakki
中科院分区：
文献类型：
--
作者：
Evran, Sefika;Tayyarcan, Emine Kubra;Boyaci, Ismail Hakki
抗生素耐药性是世界上最严重的健康问题之一。因此，寻找新的治疗方法作为抗生素的替代品已成为研究人员的优先事项。与抗生素类似，某些含有抗菌成分的产品（如糖蜜）被广泛用于消除耐药菌。糖蜜对细菌细胞有很强的抗菌作用，这种作用被认为是由于糖蜜中多酚类物质的细胞质膜和细胞蛋白质的破坏。在本研究中，研究了噬菌体-糖蜜相互作用，以检查伴随使用的影响。发现糖蜜样品使噬菌斑的大小增加了3倍，并且糖蜜的MIC和1/2 x MIC浓度增加了噬菌斑的爆发大小。虽然没有发现噬菌体和糖蜜之间的协同作用，但证明了组分的抗菌活性和糖蜜对噬菌体活性的影响。
Resistance to antibiotics is one of the most critical health problems in the world. Therefore, finding new treatment methods to be used as alternatives to antibiotics has become a priority for researchers. Similar to phages, certain products containing antimicrobial components, such as molasses, are widely used to elimi-nate resistant bacteria. Molasses has a strong antimicrobial effect on bacterial cells, and this effect is thought to be due to the breakdown of the cytoplasmic cell membrane and cell proteins of the polyphenols in molas-ses. In the present study, phage-molasses interactions were investigated to examine the effects of concomi-tant use. It was found that molasses samples increased the size of phage plaques by up to 3-fold, and MIC and 1/2 x MIC concentrations of molasses increased the burst size of phages. Although no synergistic effect was found between the phage and molasses, the antimicrobial activities of the components and the effect of mo-lasses on phage activity were demonstrated.