CHARGE DISPLACEMENT INDUCED BY RAPID STRETCH IN THE BASOLATERAL MEMBRANE OF THE GUINEA-PIG OUTER HAIR CELL
CHARGE DISPLACEMENT INDUCED BY RAPID STRETCH IN THE BASOLATERAL MEMBRANE OF THE GUINEA-PIG OUTER HAIR CELL
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DOI：
10.1098/rspb.1994.0035
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发表时间：
1994-03-22
影响因子：
4.7
通讯作者：
ASHMORE, JF
中科院分区：
文献类型：
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作者：
GALE, JE;ASHMORE, JF
在全细胞膜片钳下研究耳蜗外毛细胞基底膜的特性，以测量机械变形引起的电流和电容的变化。沿着细胞轴拉伸外毛细胞膜产生了瞬时的内向电流，随后膜的松弛产生了类似的瞬时外向电流。这些机械激活电流是速度依赖性的，平均灵敏度为29pA S mm-1。与离子电流不同，这些电流并没有反转，而是在-33 mV时达到峰值。拉伸电池还导致可测量的电容下降0.3-1.1pF-1。这些结果表明，膜拉伸可以通过逆转外毛细胞的机电转导过程而诱导快速的电荷运动。
The properties of the basolateral membrane of cochlear outer hair cells were studied under whole-cell patch clamp to measure currents and capacitance changes associated with mechanical deformation. Stretching the membrane of outer hair cells along the cell axis generated a transient inward current, and subsequent relaxation of the membrane produced a similar transient outward current. These mechanically activated currents were velocity dependent with a mean sensitivity of 29 pA s mm-1. Unlike ionic currents, these currents did not reverse, but reached a peak magnitude at -33 mV. Stretching the cell also resulted in a measurable capacitance decrease of 0.3-1.1 pF mum-1. These results suggest that membrane stretch can induce a rapid charge movement resulting from the reversal of the electromechanical transduction process in outer hair cells.