HAHA - nothing to laugh about. Measuring the immunogenicity (human anti-human antibody response) induced by humanized monoclonal antibodies applying ELISA and SPR technology
HAHA - nothing to laugh about. Measuring the immunogenicity (human anti-human antibody response) induced by humanized monoclonal antibodies applying ELISA and SPR technology
复制标题
DOI：
10.1016/j.jpba.2009.07.013
复制
发表时间：
2010-01-05
影响因子：
3.4
通讯作者：
Nechansky, Andreas
中科院分区：
文献类型：
--
作者：
Nechansky, Andreas
被动应用蛋白质诱导的免疫原性是一个严重的问题，因为它直接关系到患者的安全。对治疗性蛋白质的免疫反应的结果可以从没有任何临床意义的抗体的短暂出现到严重的危及生命的状况。本文比较了酶联免疫吸附试验（ELISA）和表面等离子体共振（SPR）两种检测免疫原性的方法，并讨论了其优缺点。(C)2009 Elsevier B.V.保留所有权利。
Immunogenicity induced by passively applied proteins is a serious issue because it is directly related to the patient's safety. The out-come of an immune reaction to a therapeutic protein can range from transient appearance of antibodies without any clinical significance to severe life threatening conditions. Within this article, enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) methodology to measure immunogenicity are compared and the pros and cons are discussed. (C) 2009 Elsevier B.V. All rights reserved.