Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis
Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis
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DOI：
10.1111/j.1744-7917.2010.01333.x
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发表时间：
2010-10-01
期刊：
影响因子：
4
通讯作者：
Guo, Yu-Yuan
中科院分区：
文献类型：
--
作者：
Yu, Hong-Kun;Chen, Hao;Guo, Yu-Yuan
二化螟是亚洲、北非和南欧地区重要的水稻害虫之一。实验室培育的转Bt基因水稻对该害虫和其他鳞翅目害虫具有良好的抗性，为该害虫的防治提供了一种可能的替代工具。克隆了编码氨基肽酶N(CsAPN)和钙粘附素(CSCAD)的全长cDNAs。在其推导的氨基酸序列中，CsAPN显示出与其他昆虫APN的共同特征和高度同源性。尽管GenBank已报道了一个编码类钙粘素蛋白的全长cDNA，但新分离的CSCAD的氨基酸序列存在一些差异，特别是在第7个钙粘素重复区域(CR7)，这表明新分离的CSCAD可能是另一个等位基因。CsAPN和CSCAD在昆虫TN细胞中成功表达，印迹分析表明这两种蛋白均能与Bt毒素Cry1Ab结合。这些结果将为研究该害虫的毒素作用方式和可能的毒素抗性机制提供有价值的信息。
The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N (CsAPN) and a cadherin (CsCad) were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here (CsCad) showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region (CR7), which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin Cry1Ab. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest.