Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana extracts
Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana extracts
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DOI：
10.1016/j.ijpharm.2013.05.012
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发表时间：
2013-08-16
影响因子：
5.8
通讯作者：
Opanasopit, Praneet
中科院分区：
文献类型：
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作者：
Charernsriwilaiwat, Natthan;Rojanarata, Theerasak;Opanasopit, Praneet
本研究的目的是制备电纺壳聚糖基纤维毡，并将山竹果船体（GM）提取物掺入到纤维毡中。选择壳聚糖-乙二胺四乙酸/聚乙烯醇（CS-EDTA/PVA）作为聚合物。将含有1、2和3重量% α-倒捻子素的GM提取物掺入CS-EDTA/PVA溶液中并静电纺丝以获得纳米纤维。使用扫描电子显微镜（SEM）分析毡的形态和直径。研究了其力学性能和溶胀性能。使用高效液相色谱法（HPLC）测定GM提取物的量。评价了其抗氧化性、抗菌性、浸提物释放性和稳定性。还在Wistar大鼠中进行了体内伤口愈合试验。结果表明，纤维的直径在纳米级上，并且在任何浓度下在垫中均未观察到提取物的晶体。所述垫提供合适的拉伸强度和溶胀性能。所有的垫子都表现出抗氧化和抗菌活性。在伤口愈合测试期间，与对照（纱布覆盖的）相比，垫加速了愈合速率。这些垫子在3个月内保持90%的α-倒捻子素含量。综上所述，利用静电纺丝法成功制备了负载转基因提取物的壳聚糖基复合材料。这些含有转基因提取物的复合材料可能为加速伤口愈合提供一个很好的选择。(C)2013年爱思唯尔B。V.保留所有权利。
The aim of this study was to prepare electrospun chitosan-based nanofiber mats and to incorporate the fruit hull of Garcinia mangostana (GM) extracts into the mats. Chitosan-ethylenediaminetetraacetic acid/polyvinyl alcohol (CS-EDTA/PVA) was selected as the polymers. The GM extracts with 1, 2 and 3 wt% alpha-mangostin were incorporated into the CS-EDTA/PVA solution and electrospun to obtain nanofibers. The morphology and diameters of the mats were analyzed using scanning electron microscopy (SEM). The mechanical and swelling properties were investigated. The amount of GM extracts was determined using high-performance liquid chromatography (HPLC). The antioxidative activity, antibacterial activity, extract release and stability of the mats were evaluated. In vivo wound healing tests were also performed in Wistar rats. The results indicated that the diameters of the fibers were on the nanoscale and that no crystals of the extract were observed in the mats at any concentration. The mats provided suitable tensile strength and swelling properties. All of the mats exhibited antioxidant and antibacterial activity. During the wound healing test, the mats accelerated the rate of healing when compared to the control (gauze-covered). The mats maintained 90% of their content of alpha-mangostin for 3 months. In conclusion, the chitosan-based nanofiber mats loaded with GM extracts were successfully prepared using the electrospinning method. These nanofiber mats loaded with GM extracts may provide a good alternative for accelerating wound healing. (C) 2013 Elsevier B. V. All rights reserved.