ISOLATION AND CHARACTERIZATION OF THE RAM SPERMATIDAL NUCLEAR PROTEIN-P1, PROTEIN-3 AND PROTEIN-T
ISOLATION AND CHARACTERIZATION OF THE RAM SPERMATIDAL NUCLEAR PROTEIN-P1, PROTEIN-3 AND PROTEIN-T
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DOI：
10.1016/0014-4827(85)90490-2
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发表时间：
1985-01-01
影响因子：
3.7
通讯作者：
LOIR, M
中科院分区：
文献类型：
--
作者：
DUPRESSOIR, T;SAUTIERE, P;LOIR, M
从非圆形精子细胞核中分离到绵羊精子细胞蛋白P1、3和T，并进行了氨基酸分析。精子蛋白是一种富含精氨酸和半胱氨酸的小碱性蛋白。蛋白质P1和T含有异常丰富的丝氨酸，其中P1的组氨酸含量特别高。测定了将这些蛋白质从精子细胞核中解离所需的氯化钠摩尔浓度。这些蛋白质只存在于精子细胞染色质的重组过程中。
Ram spermatidal proteins P1, 3 and T were isolated from non-round spermatid nuclei and characterized by amino acid analysis. Spermatidal proteins are small arginine- and cysteine-rich basic proteins. Proteins P1 and T are unusually rich in serine and the histidine content of P1 is particularly high. The NaCl molarities required to dissociate these proteins from the spermatid nuclei were determined. These proteins are present only during the reorganization of the spermatid chromatin.