Colchicine-Induced Polyploidy in Chlamydomonas.
Colchicine-Induced Polyploidy in Chlamydomonas.
复制标题
秋水仙碱诱导的衣藻多倍体。
DOI：
10.1126/science.124.3210.25
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发表时间：
1956
期刊：
影响因子：
56.9
通讯作者：
R. Krauss
中科院分区：
文献类型：
--
作者：
D. Wetherell;R. Krauss
虽然生物碱秋水仙碱已被证明是高等植物多倍体诱导的有效剂，但在微生物中尚未取得类似的成功。在最近的文献综述中，Eigsti(1)得出结论，秋水仙碱在微生物中诱导多倍体没有实质性的证据。然而，Tschermak(2)的一篇论文（未在Eigsti的综述中列出）报道了秋水仙碱处理后雌花培养物中染色体数目加倍的有丝分裂中期。建立适合遗传分析的微生物多倍体菌株，对多倍体生理学的研究具有重要意义。尽管先前文献报道的尝试不成功(3)，一系列秋水仙碱处理(4)作为衣藻遗传研究的一部分。采用标准微生物斑点板技术，将单倍体秋水仙碱暴露于浓度为0.01、0.1和1.0的秋水仙碱中。基本上没有
Although the alkaloid colchicine has proved to be an effective agent for the induction of polyploidy in higher plants, comparable success has not been achieved with microorganisms. In a recent review of the literature, Eigsti (1) concluded that no substantial evidence existed for the induction of polyploidy in microorganisms with colchicine. However, a pa-per by Tschermak (2), not listed in Eigsti's review, reported mitotic meta-phases with the doubled chromosome number in cultures of Oedogonium after treatment with colchicine. The establishment of polyploid strains of microorganisms suitable for genetic analysis would be of considerable signifi-cance for studies of the physiology of polyploidy. In spite of previous unsuc-cessful attempts reported in the literature (3), a series of colchicine treatments was made (4) as part of a larger study of in-heritance in Chlamydomonas. Haploid vegetative cells of C. euga-metos Moewus were exposed to concen-trations of 0.01, 0.1, and 1.0-percent colchicine using standard microbiological spot-plate technique. Essentially no in-