Expression Pattern of Two Collagen Type 2 α1 Genes in the Japanese Inshore Hagfish (Eptatretus burgeri) With Special Reference to the Evolution of Cartilaginous Tissue
Expression Pattern of Two Collagen Type 2 α1 Genes in the Japanese Inshore Hagfish (Eptatretus burgeri) With Special Reference to the Evolution of Cartilaginous Tissue
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DOI：
10.1002/jez.b.21322
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发表时间：
2010-03-15
影响因子：
2.2
通讯作者：
Kuratani, Shigeru
中科院分区：
文献类型：
--
作者：
Ota, Kinya G.;Kuratani, Shigeru
胶原2型α 1（Col 2A 1）蛋白是脊椎动物软骨细胞外基质（ECM）的主要成分。在过去的二十年里，Col 2A 1的进化起源已在生物化学和分子水平上研究了现存的无颌脊椎动物（盲鳗和七鳃鳗）。虽然这些研究有助于我们了解ECM蛋白的进化，但Col 2A 1基因在盲鳗中的表达谱尚未完全描述。我们进行了分子克隆和分析的Col 2A 1基因在日本近海八目鳗（Eptatretus burgeri）的基因表达模式。我们成功地分离了两个Col 2A 1基因，EbCol 2A 1A和EbCol 2A 1B，其中EbCol 2A 1A在非软骨结缔组织中表达，而EbCol 2A 1B在一些软骨元件中检测到。在此基础上，我们讨论了Col 2A 1基因在早期脊椎动物中的进化历史。J. Exp. Zool.（Mol. Dev Evol.）314 B：157-765，2070。(C)2009 Wiley-Liss，Inc.
Collagen type 2 alpha 1 (Col2A1) protein is a major component of the cartilaginous extracellular matrix (ECM) in vertebrates. Over the past two decades, the evolutionary origin of Col2A1 has been studied at the biochemical and molecular levels in extant jawless vertebrates (hagfishes and lampreys). Although these studies have contributed to our understanding of ECM protein evolution, the expression profile of the Col2A1 gene in hagfishes has not been fully described. We have performed molecular cloning and analyzed the gene expression pattern of the Col2A1 gene in the Japanese inshore hagfish (Eptatretus burgeri). We succeeded in isolating two Col2A1 genes, EbCol2A1A and EbCol2A1B, in which EbCol2A1A was expressed in the noncartilaginous connective tissues whereas EbCol2A1B was detected in some cartilaginous elements. Based on these results, we discuss the evolutionary history of Col2A1 genes in early vertebrates. J. Exp. Zool. (Mol. Dev Evol.) 314B:157-765, 2070. (C) 2009 Wiley-Liss, Inc.