Cancer stem cells in pediatric sarcomas.
Cancer stem cells in pediatric sarcomas.
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DOI：
10.3389/fonc.2013.00168
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发表时间：
2013
影响因子：
4.7
通讯作者：
Dela Cruz FS
中科院分区：
文献类型：
--
作者：
Dela Cruz FS
肉瘤代表了一组临床和生物学多样性的恶性结缔组织肿瘤。尽管积极的常规治疗，大部分肉瘤患者经历疾病复发，这将最终导致死亡。被称为癌症干细胞（CSC）的独特细胞群体的存在被认为是对当前化疗的难治性反应的原因，也是疾病转移和复发的基础--被称为CSC假说的临床推论。CSC的存在已被认为是在各种血液和实体恶性肿瘤，只是最近在肉瘤。基于我们目前对正常干细胞生物学的理解和从恶性造血和实体瘤研究中获得的证据，研究人员已经确定了候选细胞表面标志物（CD 133，CD 117，Stro-1），生化标志物（乙醛脱氢酶活性）和细胞学特征（侧群和球形集落形成），可以识别推定的肉瘤CSC。在这篇综述中，我们探讨了目前的证据表明，可能存在的肉瘤CSC。我们目前的研究在骨肉瘤，尤文氏肉瘤家族的肿瘤，横纹肌肉瘤，以及其他肉瘤亚型描述常用的分子和生化标记，以及技术，用于识别，分离和表征的候选肉瘤CSC。我们还将讨论目前的争议和面临的挑战，在肉瘤CSC的研究。
Sarcomas represent a clinically and biologically diverse group of malignant connective tissue tumors. Despite aggressive conventional therapy, a large proportion of sarcoma patients experience disease recurrence which will ultimately result in mortality. The presence of a unique population of cells, referred to as cancer stem cells (CSCs), have been proposed to be responsible for refractory responses to current chemotherapies as well underlying the basis for metastasis and relapse of disease – clinical corollaries to what has been termed the CSC hypothesis. The presence of CSCs have been suggested in a variety of hematologic and solid malignancies, and only more recently in sarcomas. Based on our current understanding of normal stem cell biology and evidence obtained from the study of malignant hematopoietic and solid tumors, researchers have identified candidate cell surface markers (CD133, CD117, Stro-1), biochemical markers (aldehyde dehydrogenase activity), and cytological characteristics (side population and spherical colony formation) that may identify putative sarcoma CSCs. In this review, we explore the current state of evidence that may suggest the existence of sarcoma CSCs. We present research in osteosarcoma, the Ewing’s sarcoma family of tumors, rhabdomyosarcoma, as well as other sarcoma subtypes to describe commonly used molecular and biochemical markers, as well as techniques, used in the identification, isolation, and characterization of candidate sarcoma CSCs. We will also discuss the current controversies and challenges that face research in sarcoma CSC.