EXPRESSION OF CYR61, A GROWTH FACTOR-INDUCIBLE IMMEDIATE-EARLY GENE
EXPRESSION OF CYR61, A GROWTH FACTOR-INDUCIBLE IMMEDIATE-EARLY GENE
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DOI：
10.1128/mcb.10.7.3569
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发表时间：
1990-07-01
影响因子：
5.3
通讯作者：
LAU, LF
中科院分区：
文献类型：
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作者：
OBRIEN, TP;YANG, GP;LAU, LF
小鼠3 T3成纤维细胞中一组被血清或纯化的血小板衍生生长因子快速激活的即刻早期基因先前已被鉴定。在这些基因中，有几个与已知或推定的转录因子和生长因子相关，支持这些基因中的一些编码对细胞生长重要的调节分子的观点。我们在这里显示，这组基因的一个成员，p361（最初确定其cDNA 3CH 61），编码一个379个氨基酸的多肽富含半胱氨酸残基。BMP 61可以通过蛋白激酶C依赖性和非依赖性途径诱导。与许多瞬时表达的即刻早期基因不同，G 0/G1过渡期到G1中期，G1 mRNA积累。这种表达模式是由于持续转录，而mRNA在G 0/G1转换期间和G1中期以相同的速率快速翻转。在生长缓慢的细胞中，mRNA水平在整个细胞周期中是恒定的。Cyr 61含有N-末端分泌信号序列;然而，通过免疫沉淀在培养基中未检测到Cyr 61。Cyr 61在血清刺激后1至2小时合成最多，并且在细胞内具有短的半衰期。
A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle. Cyr61 contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell.