5-Halogenated Thymidine Analogues Induce a Senescence-like Phenomenon in HeLa Cells
5-Halogenated Thymidine Analogues Induce a Senescence-like Phenomenon in HeLa Cells
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5-卤代胸苷类似物在 HeLa 细胞中诱导衰老样现象
DOI：
10.1271/bbb.66.877
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Ayusawa
中科院分区：
文献类型：
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作者：
E. Michishita;Naoto Matsumura;Toshihiro Kurahashi;Toshikazu Suzuki;H. Ogino;M. Fujii;D. Ayusawa
我们测试了各种胸苷类似物诱导HeLa细胞中的衰老样现象。CldU、BrdU和IdU类似地诱导衰老细胞和典型衰老标记物的形态。胸苷类似物以外的5-卤代形式只造成细胞死亡。BrdU与光照射和Hoechst 33258的短暂处理合作有效地杀死细胞，但CldU根本没有。5-因此，卤代胸苷类似物被证明是哺乳动物细胞中细胞衰老的特异性诱导剂。
We tested various thymidine analogues for induction of a senescence-like phenomenon in HeLa cells. CldU, BrdU, and IdU similarly induced the morphology of senescent cells and typical senescence markers. Thymidine analogues other than 5-halogenated forms caused only cell death. BrdU efficiently killed the cells in cooperation with irradiation with light and a brief treatment with Hoechst 33258, but CldU did not at all. 5-Halogenated thymidine analogues were thus shown to be specific inducers of cellular senescence in mammalian cells.