Potentiality of combined hepatocellular and intrahepatic cholangiocellular carcinoma originating from a hepatic precursor cell: Immunohistochemical evidence
Potentiality of combined hepatocellular and intrahepatic cholangiocellular carcinoma originating from a hepatic precursor cell: Immunohistochemical evidence
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DOI：
10.1016/j.hepres.2005.01.012
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发表时间：
2005-05-01
影响因子：
4.2
通讯作者：
Kubo, S
中科院分区：
文献类型：
--
作者：
Tanaka, S;Yamamoto, T;Kubo, S
假设肝细胞癌和肝内胆管细胞癌起源于肝干细胞，我们利用肝干细胞标记物进行了一系列免疫组织化学研究，以更好地了解肿瘤的起源。特别地，我们研究了 15 个经证实对抗肝细胞石蜡 1、抗细胞角蛋白 7 和 19 抗体具有免疫反应的组合肿瘤。宏观研究显示，15 个肿瘤中有 11 个的癌细胞形态介于肿瘤性肝细胞和胆管细胞之间（具有中间形态的组合肿瘤）。其余四个缺乏这些细胞的肿瘤被认为是普通的联合肿瘤。我们使用 20 例肝细胞癌和 10 例胆管癌作为对照。使用干细胞标记物（c-kit 和 CD34）进行免疫组织化学分析。在 15 个组合肿瘤中，8 个进行 c-kit 染色（4 个普通肿瘤中的 2 个和 11 个中间形态肿瘤中的 6 个）。两个普通组合肿瘤中的两种肿瘤细胞类型均进行 c-kit 染色。 c-kit 染色存在于所有三种形态类型的细胞中，包括所有六个中间组合肿瘤中的中间细胞。组合肿瘤中的细胞均未表现出对 CD34 的免疫反应性。 30 个对照肿瘤均不表达 CD 34 或 c-kit。目前的研究表明，肝细胞癌和肝内胆管细胞癌并非起源于肝干细胞，而是起源于肝前体细胞，例如赫林细胞管。或者，这些肿瘤可能表现出与血管生成相关的 c-kit 上调。 (c) 2005 Elsevier B.V. 保留所有权利。
Hypothesizing that combined hepatocellular and intrahepatic cholangiocellular carcinoma originates from hepatic stem cells, we conducted a series of imnumohistochemical studies using hepatic stem cell markers to better understand the origin of the tumor. Specially, we investigated 15 combined tumors confirmed to be immunoreactive to anti-hepatocyte paraffin 1, and anti-cytokeratin 7 and 19 antibodies. Macroscopic investigation revealed cancer cells morphologically intermediate between neoplastic hepatocytes and cholangiocytes (combined tumor with intermediate morphology) in 11 of 15 tumors. The remaining four tumors lacking these cells were considered ordinary combined tumors. We used 20 hepatocellular carcinomas and 10 cholangiocarcinomas as controls. Immunohistochemical analysis was performed using stem cell markers (c-kit and CD34). Of the 15 combined tumors, 8 stained for c-kit (2 of the 4 ordinary tumors and 6 of the 11 intermediate morphologic tumors). Both of the tumor cell types in both of the ordinary combined tumors stained for c-kit. The c-kit staining was present in cells of all three morphologic types, including the intermediate cells, in all six of the intermediate combined tumors. None of the cells in the combined tumors showed immunoreactivity for CD34. None of the 30 control tumors expressed CD 34 or c-kit. The present study suggests that combined hepatocellular and intrahepatic cholangiocellular carcinomas originate not from a hepatic stem cell, but from a hepatic precursor cell, such as the canal of Hering cell. Alternatively, these tumors might show up-regulation of c-kit in relation to angiogenesis. (c) 2005 Elsevier B.V. All rights reserved.