IMMUNOHISTOCHEMICAL LOCALIZATION OF A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR IN MAMMALIAN BRAIN
IMMUNOHISTOCHEMICAL LOCALIZATION OF A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR IN MAMMALIAN BRAIN
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DOI：
10.1073/pnas.84.23.8697
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
HAWROT, E
中科院分区：
文献类型：
--
作者：
DEUTCH, AY;HOLLIDAY, J;HAWROT, E
使用针对来自鱼雷电器官的纯化乙酰胆碱受体产生的单克隆抗体来免疫组织化学定位神经元烟碱乙酰胆碱受体。用该抗体染色的大鼠大脑区域与显示[3H]尼古丁结合位点的大脑区域平行，并且对应于编码α的mRNA的区域。存在神经元烟碱乙酰胆碱受体的亚基。因此，前腹侧丘脑、皮质、海马、内侧缰核、脚间核和黑质/腹侧被盖区表现出显着的免疫反应性。内侧缰核和黑质的神经元被密集染色，并且突起被明显描绘。此外，在内侧缰核（脚间核和中缝）的投影区域中，轴突具有强烈的免疫反应性，并分布到目标部位的不同细分区域。目前的数据表明，存在几个离散的神经元系统，其中烟碱乙酰胆碱受体具有重要的功能。这些免疫组织化学研究在单细胞水平上描绘了某些烟碱乙酰胆碱受体在哺乳动物中枢神经系统内的定位。
A monoclonal antibody generated against purified acetylcholine receptor from Torpedo electric organ was used to immunohistochemically localize a neuronal nicotinic acetylcholine receptor. Regions of the rat brain stained with this antibody paralleled those areas of the brain exhibiting [3H]nicotine binding sites and corresponded to areas in which mRNAs encoding for .alpha. subunits of the neuronal nicotinic acetylcholine receptor are present. Thus, the anteroventral thalamus, cortex, hippocampus, medial habenula, interpeduncular nucleus, and substantia nigra/ventral tegmental area exhibited significant immunoreactivity. Neurons of the medial habenula and substantia nigra were densely stained, and processes were prominently delineated. Furthermore, in the projection areas of the medial habenula (interpeduncular nucleus and median raphe) axons were strongly immunoreactive and were distributed to distinct subdivisions of the target sites. The present data suggest that there are several discrete neuronal systems in which nicotinic acetylcholine receptors have functional importance. These immunohistochemical studies delineate at the single-cell level the localization within the mammalian central nervous system of certain nicotinic acetylcholine receptors.