Lipopolysaccharide challenge causes exaggerated fever and increased hepatic lipopolysaccharide uptake in vinblastine-induced leukopenic guinea pigs.
Lipopolysaccharide challenge causes exaggerated fever and increased hepatic lipopolysaccharide uptake in vinblastine-induced leukopenic guinea pigs.
复制标题
脂多糖挑战会导致长春碱诱导的白细胞减少豚鼠过度发热并增加肝脏脂多糖摄取。
DOI：
10.1097/01.ccm.0000142703.76671.1c
复制
发表时间：
2004
影响因子：
8.8
通讯作者：
Blatteis,ClarkM
中科院分区：
文献类型：
--
作者：
Li,Zhonghua;Feleder,Carlos;Blatteis,ClarkM
目的：为了更好地了解免疫功能低下的患者在接受化疗后出现中性粒细胞减少和细菌感染的发热的病理生理机制。设计：前瞻性动物研究。背景：一所医学院的生理学实验室。措施和主要结果：我们用长春花碱(0.70 mg/kg，静脉注射，前4天)诱导豚鼠白细胞减少，并测定动物对2μg/kg脂多糖的发热反应和库普弗细胞摄取75μg异硫氰酸盐标记的脂多糖/kg。白血球减少动物的发热和肝脏荧光素异硫氰酸酯-内毒素摄取显著高于其controls.Conclusion:Lipopolysaccharide-challenged，长春花碱诱导的白血球减少豚鼠，表现出高热和库普弗细胞对内毒素摄取的显著增加，库普弗细胞是热原介质的主要来源。这可以解释“发烧的中性粒细胞减少症”。
Objective:To better understand the pathophysiology of the fever often manifested by immunocompromised patients undergoing chemotherapy that become neutropenic and suffer a bacterial infection.Design:Prospective animal study.Setting:A physiology laboratory in a medical school setting.Measurements and Main Results:We induced leukopenia in guinea pigs with vinblastine (0.7 mg/kg, intravenously, 4 days before) and measured the animals’ febrile response to 2 μg of lipopolysaccharide/kg and the uptake of 75 μg of fluorescein isothiocyanate-labeled lipopolysaccharide/kg by Kupffer cells. The leukopenic animals exhibited significantly higher fevers and greater hepatic fluorescein isothiocyanate-lipopolysaccharide uptake than their controls.Conclusion:Lipopolysaccharide-challenged, vinblastine-induced leukopenic guinea pigs exhibit hyperpyrexia and significantly elevated uptake of lipopolysaccharide by Kupffer cells, the major source of pyrogenic mediators. This could explain “febrile neutropenia.”