Compromised liver mitochondrial function and complex activity in low feed efficient broilers are associated with higher oxidative stress and differential protein expression
Compromised liver mitochondrial function and complex activity in low feed efficient broilers are associated with higher oxidative stress and differential protein expression
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DOI：
10.1093/ps/84.6.933
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发表时间：
2005-06-01
期刊：
影响因子：
4.4
通讯作者：
Bottje, W
中科院分区：
文献类型：
--
作者：
Iqbal, M;Pumford, NR;Bottje, W
肉鸡生长和效率的变化部分是由于肉鸡线粒体功能和生物化学的差异。为了进一步了解这一点，我们进行了两个实验，以确定a）线粒体功能和各种电子传递链（ETC）复合物的活性之间的关系，B）活性氧（ROS）H2 O2的产生及其与蛋白质氧化的关系;以及c）具有低或高饲料效率的单系雄性肉鸡（FE，每组n = 5至8）的肝脏中的线粒体蛋白质表达。线粒体功能和配合物的活动进行了测定极谱和电化学，分别。H2 O2荧光测定，而氧化蛋白（羰基）和特定的线粒体蛋白进行了分析，使用蛋白质印迹。线粒体功能（ETC耦合）和ETC复合物（I，II，III和IV）的活动高FE肉鸡相比，低FE。H_2O_2和蛋白质羰基在低铁肉鸡的肝脏高于高铁肉鸡。而4种免疫反应蛋白[NAD 3（复合物I），亚单位VII（复合物III）、细胞色素c氧化酶亚基（考克斯）II和考克斯IVb（复合物IV）]在低FE肝线粒体和2种蛋白[亚基70（复合物II）和a-ATP合酶（复合物V）]在高FE鸟类中更高，在其他18种线粒体蛋白的表达中，组间没有差异。总之，在低FE肉鸡氧化应激的增加所造成的，或可能有助于线粒体功能（ETC耦合和复杂的活动）的差异，或在肝脏中的一些线粒体蛋白质的稳态水平的差异表达。了解氧化应激在低能量肉鸡中的作用将为了解饲料效率的细胞基础提供线索。
Variations in broiler growth and efficiency have been explained in part by differences in mitochondrial function and biochemistry in broilers. To further our knowledge in this regard, 2 experiments were carried out to determine the relationships of a) mitochondrial function and activities of various electron transport chain (ETC) complexes; b) production of H2O2, a reactive oxygen species (ROS), and its association with protein oxidation; and c) mitochondrial protein expression in liver of a single line male broilers with low or high feed efficiency (FE, n = 5 to 8 per group). Mitochondrial function and complex activities were measured polarographically and spectrophotometrically, respectively. H2O2 was measured fluorimetrically, whereas oxidized protein (carbonyls) and specific mitochondrial proteins were analyzed using Western blots. Mitochondrial function (ETC coupling) and activities of ETC complexes (I, II, III, and IV) were higher in high FE compared with low FE broilers. H2O2 and protein carbonyls were higher in the livers of low FE broilers than in high FE broilers. Whereas the expression of 4 immunoreactive proteins [NAD3 (complex I), subunit VII (complex III), cytochrome c oxidase subunits (COX) II, and COX IVb (complex IV)] were higher in low FE liver mitochondria and 2 proteins [subunit 70 (complex II) and a-ATP synthase (complex V)] were higher in high FE birds, there were no differences between groups in the expression of 18 other mitochondrial proteins. In conclusion, increases in oxidative stress in low FE broilers were caused by or may contribute to differences in mitochondrial function (ETC coupling and complex activities) or the differential expression of steady-state levels of some mitochondrial proteins in the liver. Understanding the role of oxidative stress in Low FE broilers will provide clues in understanding the cellular basis of feed efficiency.