Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants.
Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants.
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DOI：
10.1152/jappl.2000.89.1.21
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发表时间：
2000-07
影响因子：
3.3
通讯作者：
Jiankang Liu;H. C. Yeo;Eva Övervik-Douki;T. Hagen;S. Doniger;Daniel W. Chu;George A. Brooks;B. Ames
中科院分区：
文献类型：
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作者：
Jiankang Liu;H. C. Yeo;Eva Övervik-Douki;T. Hagen;S. Doniger;Daniel W. Chu;George A. Brooks;B. Ames
研究了慢性运动(8wk跑台跑)和急性运动(跑步机跑至力竭)对大鼠脑、肝、心、肾和肌肉组织氧化应激的影响。测定了氧化应激的各种生物标志物，即脂质过氧化[丙二醛(MDA)]、蛋白质氧化(蛋白质羰基水平和谷氨酰胺合成酶活性)、DNA氧化损伤(8-羟基-2‘-脱氧鸟苷)和内源性抗氧化剂(抗坏血酸、α-生育酚、谷胱甘肽、泛醌、泛醌和半胱氨酸)。主要的变化是丙二醛、抗坏血酸、谷胱甘肽、半胱氨酸和半胱氨酸。丙二醛(MDA)检测显示，脑和肝脏线粒体部分呈现氧化改变，与组织匀浆相似。我们的结果表明，大脑对急性或慢性运动的氧化应激的反应与肝脏、心脏、快肌和慢肌的反应有很大的不同；急性或慢性运动的氧化应激根据器官组织类型及其内源性抗氧化剂水平的不同而引起不同的反应。
The responses to oxidative stress induced by chronic exercise (8-wk treadmill running) or acute exercise (treadmill running to exhaustion) were investigated in the brain, liver, heart, kidney, and muscles of rats. Various biomarkers of oxidative stress were measured, namely, lipid peroxidation [malondialdehyde (MDA)], protein oxidation (protein carbonyl levels and glutamine synthetase activity), oxidative DNA damage (8-hydroxy-2'-deoxyguanosine), and endogenous antioxidants (ascorbic acid, alpha-tocopherol, glutathione, ubiquinone, ubiquinol, and cysteine). The predominant changes are in MDA, ascorbic acid, glutathione, cysteine, and cystine. The mitochondrial fraction of brain and liver showed oxidative changes as assayed by MDA similar to those of the tissue homogenate. Our results show that the responses of the brain to oxidative stress by acute or chronic exercise are quite different from those in the liver, heart, fast muscle, and slow muscle; oxidative stress by acute or chronic exercise elicits different responses depending on the organ tissue type and its endogenous antioxidant levels.