Simple sequence repeats in Cucumis mapping and map merging
Simple sequence repeats in Cucumis mapping and map merging
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DOI：
10.1139/gen-43-6-963
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发表时间：
2000-12-01
期刊：
影响因子：
3.1
通讯作者：
Katzir, N
中科院分区：
文献类型：
--
作者：
Danin-Poleg, Y;Reis, N;Katzir, N
利用34个具有多态性的SSR标记对甜瓜（Cucumis梅洛L.）和黄瓜（Cucumis sativus L.）。SSR标记在3张甜瓜图谱上（Vedrantais和PI 161375图谱上18个，Piel de Sapo和PI 161375图谱上23个，PI 414723和Dulce图谱上16个）。此外，14个标记定位在黄瓜图谱上的GY14和PI 183967。SSR被证明是随机分布在整个甜瓜和黄瓜基因组。在不同的地图中的SSR标记的映射导致交叉识别的7个连锁群在所有甜瓜地图。此外，9个SSR在甜瓜和黄瓜图谱上是共有的。SSR标记作为瓜类图谱融合和黄瓜比较定位的锚点的潜力得到了证实。
Thirty-four polymorphic simple-sequence repeats (SSRs) were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (Cucumis sativus L.). SSR markers were located on three melon maps (18 on the map of 'Vedrantais' and PI 161375, 23 on the map of 'Piel de Sapo' and PI 161375, and 16 on the map of PI 414723 and 'Dulce'). In addition, 14 of the markers were located on the cucumber map of GY14 and PI 183967. SSRs proved to be randomly distributed throughout the melon and cucumber genomes. Mapping of the SSRs in the different maps led to the cross-identification of seven linkage groups in all melon maps. In addition, nine SSRs were common to both melon and cucumber maps. The potential of SSR markers as anchor points for melon-map merging and for comparative mapping with cucumber was demonstrated.