SOMITE CHONDROGENESIS
SOMITE CHONDROGENESIS
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体节软骨形成
DOI：
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发表时间：
1973
影响因子：
7.8
通讯作者：
Ronald R. Minor
中科院分区：
文献类型：
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作者：
Ronald R. Minor
本研究使用光学和电子显微镜来比较培养体节中的软骨形成与椎体软骨形成。这些研究还描述了诱导组织（脊索和脊髓）和不同营养培养基对培养体节软骨形成的一些影响。来自第 17 阶段（54-60 小时）的鸡胚体节在有或没有诱导组织的情况下进行培养，并饲喂含有马血清 (HS) 和胚胎提取物 (EE) 或胎牛血清 (FCS) 和 F12X 氨基酸分析也用于测定椎体软骨的胶原含量，其中原纤维均匀薄（约 150 Å）且无带状。这些分析提供了强有力的证据，证明胚胎软骨基质中的细无带原纤维是胶原蛋白。这些薄的无带状胶原纤维和突出的 200-800 Å 蛋白质多糖颗粒构成了发育中的椎体软骨和培养体节中形成的软骨的结构基质成分。类似的基质成分在诱导组织脊索和脊髓周围积累。这些基质成分在结构上与胚胎纤维组织中的成分不同。诱导组织对基质的合成与这些组织与体细胞间充质的诱导相互作用有关。由于组织分离和培养过程的有害影响，许多细胞在培养的前 24 小时内在体细胞间充质中死亡。尽管存在细胞死亡，但在诱导培养物中 12 小时后，软骨形成区域仍被识别，并且在所有培养物的前 2 天，结构化基质的积累量比体内同等年龄的体细胞间充质中存在的积累量更大。在所有诱导培养物中，幸存的软骨形成区域发育成透明软骨结节，在大多数非诱导培养物中，在含有FCS和F12X的培养基中，在含有HS和EE的培养基中，细胞死亡更多，基质积累更少，软骨形成更少。诱导组织以及含有FCS和F12X的营养培养基促进细胞存活、软骨基质的合成和积累以及培养体节中软骨结节的形成。
Light and electron microscopy are used in this study to compare chondrogenesis in cultured somites with vertebral chondrogenesis These studies have also characterized some of the effects of inducer tissues (notochord and spinal cord), and different nutrient media, on chondrogenesis in cultured somites Somites from stage 17 (54–60 h) chick embryos were cultured, with or without inducer tissues, and were fed nutrient medium containing either horse serum (HS) and embryo extract (EE), or fetal calf serum (FCS) and F12X Amino acid analyses were also utilized to determine the collagen content of vertebral body cartilage in which the fibrils are homogeneously thin (ca. 150 Å) and unbanded. These analyses provide strong evidence that the thin unbanded fibrils in embryonic cartilage matrix are collagen. These thin unbanded collagen fibrils, and prominent 200–800 Å protein polysaccharide granules, constitute the structured matrix components of both developing vertebral cartilage and the cartilage formed in cultured somites Similar matrix components accumulate around the inducer tissues notochord and spinal cord. These matrix components are structurally distinct from those in embryonic fibrous tissue The synthesis of matrix by the inducer tissues is associated with the inductive interaction of these tissues with somitic mesenchyme. Due to the deleterious effects of tissue isolation and culture procedures many cells die in somitic mesenchyme during the first 24 h in culture. In spite of this cell death, chondrogenic areas are recognized after 12 h in induced cultures, and through the first 2 days in all cultures there are larger accumulations of structured matrix than are present in equivalently aged somitic mesenchyme in vivo. Surviving chondrogenic areas develop into nodules of hyaline cartilage in all induced cultures, and in most non-induced cultures fed medium containing FCS and F12X There is more cell death, less matrix accumulation, and less cartilage formed in cultures fed medium containing HS and EE. The inducer tissues, as well as nutrient medium containing FCS and F12X, facilitate cell survival, the synthesis and accumulation of cartilage matrix, and the formation of cartilage nodules in cultured somites.