Transcription regulation of human chemokine receptor CCR3: evidence for a rare TATA-less promoter structure conserved between drosophila and humans.
Transcription regulation of human chemokine receptor CCR3: evidence for a rare TATA-less promoter structure conserved between drosophila and humans.
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人类趋化因子受体 CCR3 的转录调控：果蝇和人类之间保守的罕见 TATA-less 启动子结构的证据。
DOI：
10.1006/geno.2002.6801
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发表时间：
2002
期刊：
影响因子：
4.4
通讯作者：
Michael,NelsonL
中科院分区：
文献类型：
--
作者：
Vijh,Sujata;Dayhoff,DeboraE;Wang,CarolE;Imam,Zakaria;Ehrenberg,PhilipK;Michael,NelsonL
趋化因子受体CCR3在嗜酸性粒细胞疾病的发病机制中起关键作用，是HIV-1的进入联合受体。我们在这里描述了人类基因CCR3的基因组组织和一般转录调控机制。我们鉴定了由8个外显子和7个内含子交替剪接形成的6个cDNA转录本。CCR3含有一个37bp的核心启动子结构域(相对于转录起始点的−3到+34)，没有TATA盒，但包含一个启动子序列，G位于+24，下游启动子元件位于+28到+33，这在果蝇中是常见的，但到目前为止只描述了另外两个人类基因。这些元件的突变显著减弱CCR3的转录，正如RNA polII与含有DPE的果蝇启动子结合的模型所预测的那样。这些结果为果蝇和人类基因之间依赖DPE的一般转录控制机制的功能保守提供了证据。
The chemokine receptor CCR3 has a critical function in the pathogenesis of eosinophilic diseases and is an entry co-receptor for HIV-1. We describe here the genomic organization and general transcriptional control mechanism for the human gene CCR3. We identified six cDNA transcripts formed by alternative splicing of eight exons and seven introns. CCR3 contains a 37-bp core promoter domain (−3 to +34 relative to the transcription start point) lacking a TATA box but inclusive of an initiator sequence, a G at +24, and a downstream promoter element (DPE) at +28 to +33 common for Drosophila melanogaster but heretofore described for only two other human genes. Mutation of these elements significantly attenuates CCR3 transcription, as predicted by a model of RNA pol II engagement with DPE-containing Drosophila promoters. These results provide evidence for the functional conservation of a DPE-dependent, general transcription control mechanism between Drosophila and human genes.