Cellular expression of adenosine A(2A) receptor messenger RNA in the rat central nervous system with special reference to dopamine innervated areas
Cellular expression of adenosine A(2A) receptor messenger RNA in the rat central nervous system with special reference to dopamine innervated areas
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DOI：
10.1016/s0306-4522(97)00180-2
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发表时间：
1997-10-01
期刊：
影响因子：
3.3
通讯作者：
Fredholm, BB
中科院分区：
文献类型：
--
作者：
Svenningsson, P;LeMoine, C;Fredholm, BB
用核酸探针原位杂交技术研究了腺苷A（2A）受体信使RNA在中枢神经系统的细胞分布。在背侧（即尾壳核）和腹侧（即延髓核和嗅结节）纹状体，外侧隔和一些小脑浦肯野细胞中发现了特异性表达。放射性和非放射性探针的同时检测显示，背侧和腹侧纹状体中大多数含腺苷A（2A）受体信使RNA的神经元共表达多巴胺D-2受体信使RNA和前脑啡肽原A信使RNA。然而，在沿着延髓核腹侧边缘的簇群中发现了表达腺苷A（2A）受体信使RNA的神经元的一小部分亚群，但不表达前脑啡肽原A信使RNA。只有少数表达多巴胺D-2受体或P物质信使RNA的纹状体神经元也表达腺苷A（2A）受体信使RNA。最后，在伏隔核腹侧部分和嗅结节中，主要神经元亚群表达前原脑啡肽A信使RNA，但不表达腺苷A（2A）受体信使RNA。胆碱能中间神经元不表达腺苷A（2A）受体信使RNA。因此，广泛的共定位腺苷A（2A）和多巴胺D-2受体先前描述的背侧纹状体延伸到其腹侧部分。在腹侧纹状体中也有腺苷A（2A）受体信使RNA和前脑啡肽原A信使RNA的高度共表达，但在该区域内，几个拓扑定义的神经元亚群仅表达这些转录本中的一种。侧隔中大多数含腺苷A（2A）受体信使RNA的神经元确实含有前脑啡肽原A信使RNA，而只有少数共表达多巴胺D-2受体信使RNA。尽管腺苷A（2A）受体基因的表达受到限制，并且该受体在这些区域以外的极少数细胞中表达。这些结果预示腺苷A（2A）受体不仅参与运动行为，而且参与目标导向行为。(C)1997年IBRO。出版社：Elsevier Science Ltd
The cellular distribution of adenosine A(2A) receptor messenger RNA in the central nervous system was investigated using in situ hybridization with ribonucleotide probes. A specific expression was found in the dorsal (i.e. caudate-putamen) and ventral (i.e. nucleus accumbens and olfactory tubercle) striatum, the lateral septum and in some cerebellar Purkinje cells. Simultaneous detection of radioactive and non-radioactive probes showed that the majority of adenosine A(2A) receptor messenger RNA-containing neurons in the dorsal and ventral striatum co-expressed dopamine D-2 receptor messenger RNA and preproenkephalin A messenger RNA. However, a minor sub-population of neurons expressing adenosine A(2A) receptor messenger RNA, but not preproenkephalin A messenger RNA, was found in clusters along the ventral border of the nucleus accumbens. Only a small number of striatal neurons expressing dopamine D-2 receptor or substance P messenger RNAs also expressed adenosine A(2A) receptor messenger RNA. Finally, in the ventral part of nucleus accumbens and in the olfactory tubercle a major sub-population of neurons expressed preproenkephalin A messenger RNA, but not adenosine A(2A) receptor messenger RNA. Cholinergic interneurons did not express adenosine A(2A) receptor messenger RNA. Thus, the extensive co-localization of adenosine A(2A) and dopamine D-2 receptors previously described in the dorsal striatum extends into its ventral part. There is also a high degree of co-expression of adenosine A(2A) receptor messenger RNA and preproenkephalin A messenger RNA in the ventral striatum, but within this region several topologically defined sub-populations of neurons express only one of these transcripts. A majority of the adenosine A(2A) receptor messenger RNA-containing neurons in the lateral septum did contain preproenkephalin A messenger RNA, whereas only a few co-expressed dopamine D-2 receptor messenger RNA.This detailed investigation demonstrates that most of the subcortical areas innervated by dopamine have an abundant, although restricted expression of the adenosine A(2A) receptor gene and that this receptor is expressed in very few cells outside these areas. These results predict that adenosine A(2A) receptors are involved not only in motor behaviour, but also in goal-oriented behaviours. (C) 1997 IBRO. Published by Elsevier Science Ltd.