Impact of transgenic Cry1Ac+CpTI cotton on diversity and dynamics of rhizosphere bacterial community of different root environments
Impact of transgenic Cry1Ac+CpTI cotton on diversity and dynamics of rhizosphere bacterial community of different root environments
复制标题
DOI：
10.1016/j.scitotenv.2018.05.013
复制
发表时间：
2018-10-01
影响因子：
9.8
通讯作者：
Ming, Feng
中科院分区：
文献类型：
--
作者：
Li, Peng;Li, Yongchun;Ming, Feng
研究了Bt棉不同生育期不同根际环境下根际细菌群落的多样性和动态，特别是优势菌和稀有菌对Bt棉生长的响应。qPCR分析表明，Bt棉SGK321在苗期和成铃期主根和侧根际细菌丰度存在显著差异。但Bt棉与常规棉品种相同根区的根际差异不显著。细菌总属数与优势属丰度和稀有属丰度的变化规律基本一致。3个棉花品种的根际细菌多样性在主根和侧根中存在差异，但在同一根际环境中Bt棉和常规棉的差异不显著。此外，土壤pH值与细菌群落组成的变化比Bt蛋白更相关。这些结果表明，在相同的根际环境下，Bt棉根际细菌群落对Bt蛋白含量的增加没有不同的反应。特别是，优势和稀有细菌类群的多样性和群落组成的变化，在不同的根微生境。(C)2018 Elsevier B.V.版权所有。
The objective of this study was to characterize the diversity and dynamics of rhizosphere bacterial community, especially the response of dominant and rare bacterial taxa to the cultivation of Bt cotton for different root environments at different growth stages. qPCR analyses indicated that bacterial abundances of the taproots and lateral root rhizospheres of the Bt cotton SGK321 were significantly different at seedling and bolling stages. But no significant differences were detected between the same root zones from Bt and the conventional cotton varieties. Total bacterial genera had similar pattern with dominant genera in abundance, and with rare genera in richness to the changes of bacterial community, respectively. Although the rhizosphere bacterial diversity of the three cotton varieties changed in taproot and lateral root, no significant differences were detected in the same root environments between Bt and conventional cotton. Moreover, Soil pH was more correlated with variations in the bacterial community composition than Bt proteins. In conclusion, these results revealed no indication that rhizosphere bacterial community of Bt cotton had different response to increased Bt protein regarding the same root environment. In particular, dominant and rare bacterial taxa showed the variation in diversity and community composition in different root microhabitats. (C) 2018 Elsevier B.V. All rights reserved.