Maternal intake of trans-unsaturated or interesterified fatty acids during pregnancy and lactation modifies mitochondrial bioenergetics in the liver of adult offspring in mice
Maternal intake of trans-unsaturated or interesterified fatty acids during pregnancy and lactation modifies mitochondrial bioenergetics in the liver of adult offspring in mice
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DOI：
10.1017/s0007114517001817
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发表时间：
2017-07-01
影响因子：
3.6
通讯作者：
Tavares-do-Carmo, Maria das Gracas
中科院分区：
文献类型：
--
作者：
de Velasco, Patricia C.;Chicaybam, Gustavo;Tavares-do-Carmo, Maria das Gracas
母体饮食中的膳食脂质质量会使后代在以后的生活中患上疾病。我们调查了母亲摄入棕榈油或酯交换脂肪（反式不饱和脂肪酸（FA）的替代品）是否会引起成年后代的代谢变化。在妊娠和哺乳期间，C57 BL/6雌性小鼠接受含有富含反式不饱和脂肪酸（TG）的部分氢化植物脂肪、棕榈油（PG）、酯交换脂肪（IG）或大豆油（CG）的正常脂类饮食。断奶后，所有组的雄性后代接受对照饲料，直至第110天。确定血糖和TAG和肝脏FA谱。通过高分辨率呼吸测定法测量VO 2、荧光测定法检测过氧化氢（H2 O2）产生和线粒体Ca 2+摄取来评估肝脏线粒体功能。结果表明，IG后代的血糖增加了20%，IG和TG后代的TAG分别增加了2倍和1.9倍。TG和IG仔鼠肝脏MUFA和PUFA含量均低于CG仔鼠。PG后代的肝脏MUFA含量也降低。FA组成的这些修改可能会影响肝脏线粒体功能，因为TG后代的呼吸功能受损，IG后代的H2 O2产量较高。此外，线粒体Ca 2+的保留能力分别减少了约40%和55%的TG和IG后代。总之，母亲食用反式不饱和脂肪和酯交换脂肪通过损害肝脏中的线粒体生物能量学和脂质代谢来影响后代的健康。
The quality of dietary lipids in the maternal diet can programme the offspring to diseases in later life. We investigated whether the maternal intake of palm oil or interesterified fat, substitutes for trans-unsaturated fatty acids (FA), induces metabolic changes in the adult offspring. During pregnancy and lactation, C57BL/6 female mice received normolipidic diets containing partially hydrogenated vegetable fat rich in trans-unsaturated fatty acids (TG), palm oil (PG), interesterified fat (IG) or soyabean oil (CG). After weaning, male offspring from all groups received the control diet until day 110. Plasma glucose and TAG and liver FA profiles were ascertained. Liver mitochondrial function was accessed with high-resolution respirometry by measuring VO2, fluorimetry for detection of hydrogen peroxide (H2O2) production and mitochondrial Ca2+ uptake. The results showed that the IG offspring presented a 20% increase in plasma glucose and both the IG and TG offspring presented a 2- and 1.9-fold increase in TAG, respectively, when compared with CG offspring. Liver MUFA and PUFA contents decreased in the TG and IG offspring when compared with CG offspring. Liver MUFA content also decreased in the PG offspring. These modifications in FA composition possibly affected liver mitochondrial function, as respiration was impaired in the TG offspring and H2O2 production was higher in the IG offspring. In addition, mitochondrial Ca2+ retention capacity was reduced by approximately 40 and 55% in the TG and IG offspring, respectively. In conclusion, maternal consumption of trans-unsaturated and interesterified fat affected offspring health by compromising mitochondrial bioenergetics and lipid metabolism in the liver.