Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice.
Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice.
复制标题
热应激响应AtPLC9的异源表达赋予转基因水稻耐热性
DOI：
10.1186/s12870-020-02709-5
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发表时间：
2020-11-11
影响因子：
5.3
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Liu Y;Liu X;Wang X;Gao K;Qi W;Ren H;Hu H;Sun D;Bai J;Zheng S
背景随着全球变暖的日益严重，提高农作物的耐热性是当务之急。结果在本研究中，我们在水稻中异地表达了AtPLC9，以检测其在水稻中提高耐热性的潜力。虽然AtPLC9没有提高水稻对盐、干旱或寒冷的耐受性，但转基因水稻确实比野生型表现出更强的耐热性。高通量RNA-SEQ显示了转基因植物在热胁迫后广泛和动态的转录组重组。此外，热胁迫后转基因水稻中一些转录因子和钙离子相关基因的表达出现了特异性的上调，这可能是转基因水稻耐热性增强的原因之一。结论本研究为利用AtPLC9基因改良谷类作物的耐热性提供了初步的指导，并从更广泛的意义上强调了外源转化可以辅助分子育种。
BackgroundAs global warming becomes increasingly severe, it is urgent that we enhance the heat tolerance of crops. We previously reported thatArabidopsis thalianaPHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C9 (AtPLC9) promotes heat tolerance.ResultsIn this study, we ectopically expressedAtPLC9in rice to examine its potential to improve heat tolerance in this important crop. Whereas AtPLC9 did not improve rice tolerance to salt, drought or cold, transgenic rice did exhibit greater heat tolerance than the wild type. High-throughput RNA-seq revealed extensive and dynamic transcriptome reprofiling in transgenic plants after heat stress. Moreover, the expression of some transcription factors and calcium ion-related genes showed specific upregulation in transgenic rice after heat stress, which might contribute to the enhanced heat tolerance.ConclusionsThis study provides preliminary guidance for using AtPLC9 to improve heat tolerance in cereal crops and, more broadly, highlights that heterologous transformation can assist with molecular breeding.