DIELECTROPHORETIC SEPARATION OF MAMMALIAN-CELLS STUDIED BY COMPUTERIZED IMAGE-ANALYSIS
DIELECTROPHORETIC SEPARATION OF MAMMALIAN-CELLS STUDIED BY COMPUTERIZED IMAGE-ANALYSIS
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DOI：
10.1088/0957-0233/3/5/001
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发表时间：
1992-05-01
影响因子：
2.4
通讯作者：
BECKER, FF
中科院分区：
文献类型：
--
作者：
GASCOYNE, PRC;HUANG, Y;BECKER, FF
建立了一种研究哺乳动物细胞同质群和混合群介电电泳特性的方法。采用计算机图像分析来量化悬浮在低电导率介质中的细胞的运动速率，因为它们在由叉指电极阵列产生的非均匀交变电场的影响下移动。正如介电泳所预期的，当细胞的极化率超过悬浮介质的极化率时，细胞收集在阵列的高度不均匀的电场区域，或者当细胞的极化率小于其介质的极化率时，细胞收集在远离这些区域的区域。这两种类型的行为被归类为积极或消极的介电电泳分别，和细胞收集模式同意以及与所采用的电极配置的计算。在5 × 10（2）-10（5）Hz范围内，测量了正常、白血病和分化诱导白血病小鼠红细胞的介电电泳特性随频率的变化，结果显示有显著差异。通过选择合适的频率和细胞悬浮介质，介电电泳可从细胞混合物中分离出不同类型的细胞。图像分析技术不仅可以快速表征均匀的细胞群，而且还允许在细胞混合物中区分单个亚群;这些是开发用于医学和生物技术应用的介电电泳分离方法的必要步骤。
A method was developed for studying the dielectrophoretic properties of both homogeneous and mixed populations of mammalian cells. Computerized image analysis was employed to quantify the rate of motion of cells suspended in low conductivity medium as they moved under the influence of a non-uniform alternating electric field produced by an interdigitated electrode array. As expected for dielectrophoresis, cells collected at highly inhomogeneous electric field regions of the array when the electrical polarizability of cells exceeded that of the suspending medium or away from such regions when their polarizability was less than that of their medium. These two types of behaviour are classified as positive or negative dielectrophoresis respectively, and the cell collection patterns agreed well with those computed for the electrode configuration employed. The dielectrophoretic characteristics of normal, leukaemic, and differentiation-induced leukaemic mouse erythrocytes were measured as a function of frequency in the range 5 x 10(2)-10(5) Hz and were shown to be significantly different. By suitable choices of applied frequency and cell suspension medium, dielectrophoretic separation of different cell types from cell mixtures was demonstrated. The image analysis technique not only allows for rapid characterization of homogeneous cell populations but also permits individual subpopulations to be discriminated within cell mixtures; these are necessary steps for the development of dielectrophoretic separation methodologies for medical and biotechnological applications.