Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress
Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress
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DOI：
10.1016/j.ecoenv.2008.04.024
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发表时间：
2009-02-01
影响因子：
6.8
通讯作者：
Janas, K. M.
中科院分区：
文献类型：
--
作者：
Posmyk, M. M.;Kontek, R.;Janas, K. M.
研究了Cu2+胁迫下红甘蓝幼苗体内的酚类物质：花青素(ATH)、芥子油酯和抗氧化酶：超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、愈创木酚过氧化物酶(POX)、抗坏血酸过氧化物酶(APX)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽还原酶(GR)的活性。低剂量(0.5 mM)的Cu2+能提高红甘蓝中ATH和芥子酰衍生物的含量。高浓度Cu2+(2.5 mM)可引起组织氧化应激，增加组织中硫代巴比妥酸反应物质(TBARS)的含量。TBARS水平越低，ATH含量越高。合成这些异黄酮类化合物似乎是对抗活性氧自由基(ROS)的有效策略。抗氧化酶活性分析表明，过氧化物酶是Cu2+胁迫下红甘蓝幼苗中活性最高的酶。这可能是因为酚类化合物(PHC)是抵御金属胁迫的第一道防线，它也可以是不同过氧化物酶的底物。(C)2008 Elsevier Inc.保留所有权利。
The phenolics: anthocyanin (ATH), sinapoyl esters and activity of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and glutathione reductase (GR), in red cabbage seedlings subjected to Cu2+ stress were investigated. Cu2+ at low doses (0.5 mM), increased the levels of ATH and sinapoyl derivatives in red cabbage. High Cu2+ concentration (2.5 mM) provoked oxidative stress and enhanced thiobarbituric acid reactive substances (TBARS) content in tissues. A lower level of TBARS was correlated with high ATH content. It seems that synthesis of these isoflavonoids is an effective strategy against reactive oxygen species (ROS). The analysis of the antioxidant enzymes activity suggested that peroxidases were the most active enzymes in red cabbage seedlings exposed to Cu2+ stress. It could results from the fact that phenolic compounds (PhC), which could be also substrates for different peroxidases, were the first line of defence against metal stress. (C) 2008 Elsevier Inc. All rights reserved.