Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.
Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.
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DOI：
10.1039/c000453g
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发表时间：
2010-06-07
期刊：
影响因子：
6.1
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Mudanyali O;Tseng D;Oh C;Isikman SO;Sencan I;Bishara W;Oztoprak C;Seo S;Khademhosseini B;Ozcan A
尽管光学成像技术进展迅速，但大多数先进的显微镜设备仍然需要复杂而昂贵的设置，这不幸地限制了它们在设备齐全的实验室之外的使用。与此同时，在资源有限的环境中，显微镜的要求与在先进实验室中遇到的要求有很大的不同，这种成像设备应该具有成本效益、紧凑、重量轻、适当的准确性和简单性，以便受过最低培训的人员使用。此外，理想情况下，这些便携式显微镜应该作为远程医疗网络的一部分进行数字集成，该网络将各种移动医疗保健提供者连接到中心实验室或医院。为此，我们在这里展示了一种重约46克、尺寸小于4.2 cm×4.2 cm×5.8 cm的无透镜片上显微镜，它在~24mm2的大视场内实现了亚细胞分辨率。这款体积小、重量轻的显微镜基于数字同轴全息术，不需要任何透镜、笨重的光学/机械部件或激光等相干源。取而代之的是，它利用一个简单的发光二极管(LED)和一个紧凑的光电传感器阵列来记录对象的无透镜全息图，然后允许快速数字重建对象的常规传输或差分干涉对比(DIC)图像。由于这种无透镜非相干全息显微镜的光收集效率提高了数量级，并且对机械失调非常健壮，因此它可能会提供一种经济高效的工具，特别是在资源有限的情况下涉及各种全球健康问题的远程医疗应用。
Despite the rapid progress in optical imaging, most of the advanced microscopy modalities still require complex and costly set-ups that unfortunately limit their use beyond well equipped laboratories. In the meantime, microscopy in resource-limited settings has requirements significantly different from those encountered in advanced laboratories, and such imaging devices should be cost-effective, compact, light-weight and appropriately accurate and simple to be usable by minimally trained personnel. Furthermore, these portable microscopes should ideally be digitally integrated as part of a telemedicine network that connects various mobile health-care providers to a central laboratory or hospital. Toward this end, here we demonstrate a lensless on-chip microscope weighing ~46 grams with dimensions smaller than 4.2cm × 4.2cm × 5.8cm that achieves sub-cellular resolution over a large field of view of ~24 mm2. This compact and light-weight microscope is based on digital in-line holography and does not need any lenses, bulky optical/mechanical components or coherent sources such as lasers. Instead, it utilizes a simple light-emitting-diode (LED) and a compact opto-electronic sensor-array to record lensless holograms of the objects, which then permits rapid digital reconstruction of regular transmission or differential interference contrast (DIC) images of the objects. Because this lensless incoherent holographic microscope has orders-of-magnitude improved light collection efficiency and is very robust to mechanical misalignments it may offer a cost-effective tool especially for telemedicine applications involving various global health problems in resource limited settings.
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影响因子：
3.6
作者：
FIENUP, JR
通讯作者：
FIENUP, JR
DOI：
10.1073/pnas.0900245106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Pavani, Sri Rama Prasanna;Thompson, Michael A.;Moerner, W. E.
通讯作者：
Moerner, W. E.
影响因子：
3.6
作者：
Garcia-Sucerquia, J;Xu, WB;Kreuzer, HJ
通讯作者：
Kreuzer, HJ
DOI：
10.1364/josaa.10.000423
发表时间：
1993-03-01
影响因子：
1.9
作者：
KOREN, G;POLACK, F;JOYEUX, D
通讯作者：
JOYEUX, D
影响因子：
6.1
作者：
Heng, Xin;Erickson, David;Yang, Changhuei
通讯作者：
Yang, Changhuei