Comparison between plasma and erythrocyte fatty acid content as biomarkers of fatty acid intake in US women
Comparison between plasma and erythrocyte fatty acid content as biomarkers of fatty acid intake in US women
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DOI：
10.1093/ajcn/86.1.74
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发表时间：
2007-07-01
影响因子：
7.1
通讯作者：
Hu, Frank B.
中科院分区：
文献类型：
--
作者：
Sun, Qi;Ma, Jing;Hu, Frank B.
背景资料：红细胞脂肪酸在反映长期脂肪酸摄入方面可能上级血浆脂肪酸，因为红细胞对近期摄入的敏感性较低，周转率较慢。目的：目的是比较红细胞和血浆的脂肪酸含量反映日常脂肪酸摄入的能力。设计：本文用毛细管气液色谱法测定了306名43-69岁美国妇女血浆和红细胞中的脂肪酸。结果：二十二碳六烯酸（DHA，22：6 n-3）在红细胞和血浆中与其摄入量的相关性最强，但红细胞DHA浓度[斯皮尔曼偏相关系数（r（s））= 0.56]优于血浆DHA浓度（r（s）= 0.48）。红细胞中的总反式脂肪酸（r（s）= 0.43）和总18：1反式异构体（r（s）= 0.42）与摄入量的相关性也比血浆中的相关性更强（分别为r（s）= 0.30和r（s）= 0.29）。亚油酸（18：2n-6;红细胞，r（s）= 0.24;血浆，r（s）= 0.25）、α-亚麻酸（18：3 n-3;红细胞，r（s）= 0.18;血浆，r（s）= 0.23）和二十碳五烯酸（20：5 n-3;红细胞，r（s）= 0.38;血浆，r（s）= 0.21）观察到中度相关性。对于多不饱和脂肪酸和反式脂肪酸，摄入量和生物标志物之间的相关性改善适度时，平均摄入量超过前几年used.Conclusion：红细胞n-3脂肪酸的海洋来源和反式脂肪酸含量是合适的生物标志物，长期摄入。
Background: Erythrocyte fatty acids may be superior to plasma fatty acids for reflecting long-term fatty acid intake because of less sensitivity to recent intake and a slower turnover rate.Objective: The objective was to compare the fatty acid content of erythrocytes with that of plasma with respect to their abilities to reflect usual fatty acid intake.Design: Fatty acids in plasma and erythrocytes were measured by capillary gas-liquid chromatography in 306 US women aged 43-69 y. Fatty acid intake was assessed with a food-frequency questionnaire, which was validated for measuring intakes of various fatty acids.Results: Docosahexaenoic acid (DHA, 22:6n-3) in erythrocytes and plasma provided the strongest correlations with its intake, but erythrocyte DHA concentrations [Spearman's partial correlation coefficient (r(s)) = 0.56] were better than plasma DHA concentrations (r(s) = 0.48) as a biomarker. Total trans fatty acids (r(s) = 0.43) and total 18:1 trans isomers (r(s) = 0.42) in erythrocytes were also more strongly correlated with intake than were those in plasma (r(s) = 0.30 and r(s) = 0.29, respectively). Moderate correlations were observed for linoleic acid (18:2n-6; erythrocytes, r(s) = 0.24; plasma, r(s) = 0.25), alpha-linolenic acid (18:3n-3; erythrocytes, r(s) = 0.18; plasma, r(s) = 0.23), and eicosapentaenoic acid (20:5n-3; erythrocytes, r(s) = 0.38; plasma, r(s) = 0.21). For polyunsaturated and trans fatty acids, correlations between intakes and biomarkers improved moderately when average intakes over previous years were used.Conclusion: Erythrocyte n-3 fatty acids of marine origin and trans fatty acid content are suitable biomarkers for long-term intake.