Proteomic analysis of brain proteins of rats exposed to high fluoride and low iodine
Proteomic analysis of brain proteins of rats exposed to high fluoride and low iodine
复制标题
高氟低碘暴露大鼠脑蛋白的蛋白质组学分析
DOI：
10.1007/s00204-010-0537-5
复制
发表时间：
2011-01-01
影响因子：
6.1
通讯作者：
Wang, Jundong
中科院分区：
文献类型：
--
作者：
Ge, Yaming;Niu, Ruiyan;Wang, Jundong
流行病学调查显示，高氟低碘对儿童智商有较强的不良影响。研究还报告，在一些高氟化物地区也存在缺碘现象，特别是在中国。在这里，利用蛋白质组学技术，我们首次报道了在出生后第20天暴露于高氟化物和/或低碘的后代大鼠脑蛋白质的蛋白质组学变化。为了研究这两种元素对中枢神经系统损伤的分子机制，我们分离了蛋白质并用二维凝胶电泳（2DE）对其进行了分析。通过Image-Master 2D Elite软件分析，得到处理组2DE凝胶中71个蛋白点上调或下调2倍，5个蛋白点上调或下调5倍，与对照组比较。利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定了发生5倍变化的蛋白。所鉴定的蛋白质主要与细胞信号转导、能量代谢和蛋白质代谢有关，为探索高氟低碘神经毒性机制提供了有价值的线索。此外，这些结果可以为过量氟和低碘造成的危害提供潜在的生物标志物。
Epidemiological investigations reveal that high fluoride and low iodine have strong adverse effects on the intelligence quotient (IQ) of children. Studies also report that in some high fluoride areas, iodine deficiency also exists, especially in China. Here, with the proteomic techniques, we first report on the proteomic changes in brain proteins in offspring rats at postnatal day 20 exposed to high fluoride and/or low iodine. To investigate molecular mechanisms of central neural system injury induced by the above two elements, proteins were isolated and profiled by two-dimensional gel electrophoresis (2DE). By the analysis of Image-Master 2D Elite software, 71 protein spots in 2DE gels of treatment groups were gained and up- or down-regulated by two folds, and 5 proteins were regulated by five folds, with the comparison to the control group. The proteins changed by five folds were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The identified proteins are mainly related with cellular signaling, energy metabolism, and protein metabolism and provide a valuable clue to explore the mechanism underlining the neurotoxicity of high fluoride and low iodine. Moreover, these results could provide potential biomarkers for hazards caused by excessive fluoride and low iodine.