A 101-KILODALTON HEME-BINDING PROTEIN ASSOCIATED WITH CONGO RED BINDING AND VIRULENCE OF SHIGELLA-FLEXNERI AND ENTEROINVASIVE ESCHERICHIA-COLI STRAINS
A 101-KILODALTON HEME-BINDING PROTEIN ASSOCIATED WITH CONGO RED BINDING AND VIRULENCE OF SHIGELLA-FLEXNERI AND ENTEROINVASIVE ESCHERICHIA-COLI STRAINS
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DOI：
10.1128/iai.57.11.3534-3539.1989
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发表时间：
1989-11-01
影响因子：
3.1
通讯作者：
PAYNE, SM
中科院分区：
文献类型：
--
作者：
STUGARD, CE;DASKALEROS, PA;PAYNE, SM
福氏志贺菌结合刚果红或氯化血红素的能力与毒力相关。在S.通过四甲基联苯胺染色方法鉴定弗氏菌，该方法检测聚丙烯酰胺凝胶中的血红素-蛋白质复合物。用125I标记细胞表面多肽揭示了101-kDa血红素结合蛋白在细胞表面上表达。该蛋白的表达受生长温度的调控，并被发现是由大毒力质粒编码。弗莱克斯内里。缺失突变体和Tn5插入突变体，这是阴性的刚果红结合（Crb-）不表达的101-kDa的蛋白质。肠侵袭性大肠埃希菌为Crb+，其质粒与S.弗氏菌毒力质粒，也表达101-kDa蛋白。在S. flexneri和肠侵袭性E.大肠杆菌中的表达与这些质粒的9.2-内切酶EcoRI片段的存在相关。
The ability of Shigella flexneri to bind Congo red or hemin is associated with virulence. A 101-kilodalton (kDa) protein responsible for this phenotype (Crb+) in S. flexneri was identified by a tetramethylbenzidine staining procedure which detects heme-protein complexes in polyacrylamide gels. Labeling of cell-surface polypeptides with 125I revealed that the 101-kDa heme-binding protein is expressed on the cell surface. Expression of the protein was regulated by growth temperature and was found to be encoded by the large virulence plasmid of S. flexneri. Deletion mutants and a Tn5 insertion mutant which were negative for Congo red binding (Crb-) did not express the 101-kDa protein. Enteroinvasive Escherichia coli strains that were Crb+, and whose plasmids shared homology with the S. flexneri virulence plasmid, also expressed the 101-kDa protein. Expression of the protein in S. flexneri and enteroinvasive E. coli correlated with the presence of a 9.2-kilobase EcoRI fragment of these plasmids.