Molecular weight determination of megadalton DNA electrospray ions using charge detection time-of-flight mass spectrometry
Molecular weight determination of megadalton DNA electrospray ions using charge detection time-of-flight mass spectrometry
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DOI：
10.1002/rcm.1290091513
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发表时间：
1995-01-01
影响因子：
2
通讯作者：
Benner, WH
中科院分区：
文献类型：
--
作者：
Fuerstenau, SD;Benner, WH
我们目前获得的结果与一种新型的质谱仪能够确定产生的大分子样品中的兆道尔顿（MDa）尺寸范围的多电荷电喷雾离子的质量。该仪器利用灵敏的放大器，当单个离子通过管检测器时，该放大器可以检测单个离子上的电荷。利用已知的静电能量对离子的速度测量提供了离子的质荷比。离子电荷的同时检测允许对每个离子进行质量分配。质量大于1 × 10（6）Da且电荷数（z）超过425 e（-）的DNA和聚合物分子的电喷雾离子在该质谱仪中容易检测。还测量了小颗粒的重量。轴上单离子检测配置提供了近100%的占空比，并将电喷雾质谱的实际应用扩展到使用相对便宜的仪器分析非常大的分子。
We present results obtained with a novel mass spectrometer capable of determining the mass of multiply charged electrospray ions generated from samples of macromolecules in the megadalton (MDa) size range. The instrument utilizes a sensitive amplifier which can detect the charge on a single ion as It passes through a tube detector. A velocity measurement of an ion with known electrostatic energy provides the ion's mass-to-charge ratio. Simultaneous detection of the ion charge permits a mass assignment to be made for each ion. Electrospray ions of DNA and polymer molecules with masses greater than 1 X 10(6) Da and charge numbers (z) in excess of 425 e(-) are readily detected in this mass spectrometer. The weights of small particles were also measured, The on-axis single-ion detection configuration provides a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation.