Multicolour whole-mount in situ hybridization to Drosophila embryos
Multicolour whole-mount in situ hybridization to Drosophila embryos
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DOI：
10.1007/s004270050055
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发表时间：
1996-11-01
影响因子：
2.4
通讯作者：
Gerster, T
中科院分区：
文献类型：
--
作者：
Hauptmann, G;Gerster, T
我们报告了一个扩展的整体安装在果蝇胚胎原位杂交程序。通过使用分别用地高辛、荧光素和生物素标记的探针，该方案允许以三种颜色检测来自三种不同基因的RNA。通过使用不同的显色底物组合用适当的碱性磷酸酶缀合物连续染色，并通过低pH洗涤连续去除抗体缀合物，检测杂交的探针。
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.