A comparative study of the genetic diversity of the 42kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax.
A comparative study of the genetic diversity of the 42kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax.
复制标题
恶性疟原虫和间日疟原虫裂殖子表面蛋白1 42kDa片段遗传多样性的比较研究。
DOI：
10.1016/j.meegid.2006.08.002
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Escalante,AnaniasA
中科院分区：
文献类型：
--
作者：
Pacheco,MAndreina;Poe,AmandaC;Collins,WilliamE;Lal,AltafA;Tanabe,Kazuyuki;Kariuki,SimonK;Udhayakumar,Venkatachalam;Escalante,AnaniasA
我们研究了恶性疟原虫和间日疟原虫裂殖子表面蛋白1（MSP-1）抗原的42 kDa片段的遗传多样性，以及非人灵长类疟疾寄生虫。该片段经历蛋白水解切割，产生在红细胞侵入中至关重要的19 kDa（MSP-119）和33 kDa（MSP-133）的两个片段。我们发现，无论物种如何，MSP-133片段总体上都比MSP-119片段表现出更大的遗传多样性。通过比较非同义替换与同义替换的比率，我们发现了仅在人类疟疾寄生虫中存在正自然选择的证据。此外，我们发现两种主要的人类疟疾寄生虫之间存在明显差异。在恶性疟原虫中，MSP-119区域受到正向自然选择的作用，而MSP-133区域则处于中性或纯化选择状态。在间日疟原虫中观察到相反的模式。我们的研究结果表明，在每个主要的人类疟疾寄生虫的宿主-寄生虫的免疫相互作用，这种抗原的不同作用。
We investigated the genetic diversity of the 42kDa fragment of the merozoite surface protein 1 (MSP-1) antigen in Plasmodium falciparum and P. vivax, as well as in non-human primate malarial parasites. This fragment undergoes a proteolytic cleavage generating two fragments of 19kDa (MSP-119) and 33kDa (MSP-133) that are critical in erythrocyte invasion. We found that overall the MSP-133fragment exhibits greater genetic diversity than the MSP-119regardless of the species. We have found evidence for positive natural selection only in the human malaria parasites by comparing the rate of non-synonymous versus synonymous substitutions. In addition, we found clear differences between the two major human malaria parasites. In the case of P. falciparum, positive natural selection is acting on the MSP-119region while the MSP-133is neutral or under purifying selection. The opposite pattern was observed in P. vivax. Our results suggest different roles of this antigen in the host–parasite immune interaction in each of the major human malarial parasites.