The mechanism of type IA topoisomerases
The mechanism of type IA topoisomerases
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DOI：
10.1073/pnas.132378799
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发表时间：
2002-09-17
影响因子：
11.1
通讯作者：
Croquette, V
中科院分区：
文献类型：
--
作者：
Dekker, NH;Rybenkov, VV;Croquette, V
细胞DNA的拓扑结构是由一种叫做拓扑异构酶的酶仔细控制的。通过使用单分子技术，我们监测了两个IA型拓扑异构酶的活性在真实的时间条件下，其中检测到单弛豫事件。我们观察到的超螺旋的严格的一次一个的去除确立了这些酶使用酶桥连的链通过机制，该机制非常适合于它们的生理作用，并证明了与II型拓扑异构酶的机械统一性。
The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II topoisomerases.