Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases
Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases
复制标题
DOI：
10.1016/j.enzmictec.2011.01.002
复制
发表时间：
2011-04-07
影响因子：
3.4
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Yamada, Ryosuke;Yamakawa, Syun-ichi;Kondo, Akihiko
高效产乙醇酵母酿酒酵母不能直接从生淀粉中生产乙醇。因此，传统的乙醇生产需要昂贵且复杂的过程。在这项研究中，我们开发了一种直接高效的乙醇生产工艺，利用日本收获的高产稻米，使用表达淀粉酶的酵母，无需任何预处理或添加酶或营养素。当添加营养物质时，高产糙米的乙醇生产率（1.1 g/L/h）比纯化的生玉米淀粉（0.2 g/L/h）的乙醇生产率高约5倍。使用相当于发酵体积10%的接种量且不添加任何营养物时，24小时内乙醇生产率和产量分别达到1.2 g/L/h和101%。高产稻被证明是生产生物乙醇的合适原料。此外，我们的多倍体淀粉酶表达酵母足够强大，可以从真实的生物质中有效地生产乙醇。这是使用表达淀粉酶的酵母菌株在真实生物质上直接生产乙醇的第一份报告，无需任何预处理或商业酶添加。 (C) 2011 Elsevier Inc. 保留所有权利。
Efficient ethanol producing yeast Saccharomyces cerevisiae cannot produce ethanol from raw starch directly. Thus the conventional ethanol production required expensive and complex process. In this study, we developed a direct and efficient ethanol production process from high-yielding rice harvested in Japan by using amylase expressing yeast without any pretreatment or addition of enzymes or nutrients. Ethanol productivity from high-yielding brown rice (1.1 g/L/h) was about 5-fold higher than that obtained from purified raw corn starch (0.2 g/L/h) when nutrients were added. Using an inoculum volume equivalent to 10% of the fermentation volume without any nutrient supplementation resulted in ethanol productivity and yield reaching 1.2 g/L/h and 101%, respectively, in a 24-h period. High-yielding rice was demonstrated to be a suitable feedstock for bioethanol production. In addition, our polyploid amylase-expressing yeast was sufficiently robust to produce ethanol efficiently from real biomass. This is first report of direct ethanol production on real biomass using an amylase-expressing yeast strain without any pretreatment or commercial enzyme addition. (C) 2011 Elsevier Inc. All rights reserved.