Osteoinductive effects of tantalum and titanium on bone mesenchymal stromal cells and bone formation in ovariectomized rats
Osteoinductive effects of tantalum and titanium on bone mesenchymal stromal cells and bone formation in ovariectomized rats
复制标题
钽和钛对去卵巢大鼠骨间充质基质细胞和骨形成的骨诱导作用
DOI：
10.26355/eurrev_201811_16241
复制
发表时间：
2018-11-01
影响因子：
3.3
通讯作者：
Zou, D.
中科院分区：
文献类型：
--
作者：
Lu, M-M;Wu, P-S;Zou, D.
目的：虽然钽（Ta）在健康受试者中表现出比钛（Ti）更好的骨诱导性，但其在骨质疏松症中的相对作用尚不清楚。材料和方法：本研究采用体外培养的方法，在Ta和Ti基质上接种OVX-rBMSC，用于体外评价细胞活力、活性氧（ROS）产生、碱性磷酸酶（ALP）活性，细胞外矿化成骨基因和蛋白表达参与骨形态发生蛋白（BMP 2）/小母细胞抗十肢瘫痪同源物1（Smad 1）通路。为了进行体内评估，将Ta和Ti植入物包埋在卵巢切除大鼠的股骨缺损中，随后进行连续的荧光染料标记和组织学染色。与Ti相比，Ta基底表现出更高的活细胞百分比（96.5 +/- t 0.26 vs. 88.17 +/- 2.23%），较低的ROS水平（65% vs. Ti），以及增强的ALP活性和细胞外基质钙化。逆转录-聚合酶链反应和蛋白质印迹分析验证了Ta对小母体在mRNA（1.5-2倍）和蛋白质（1.2-1.8倍）水平上对十肢瘫痪同源物1（Smad 1）、侏儒相关转录因子2、骨形态发生蛋白（BMP 2）和ALP表达的更好的骨诱导作用。BMP 2/Smad 1信号的过表达或敲低导致OVX-rBMSC在两个表面上的成骨显著增强或恶化。此外，Ta组显示出更多的新骨形成（与钛相比为1.3-1.5倍），骨-植入物接触略好（31.82 +/- 4.07 vs. 25.2-3.84%，植入后8周，p = 0.052），无特定表面结构的贡献。与T1相比，Ta对OVX-rBMSC表现出更好的生物相容性和骨诱导性，并且优先的Ta骨诱导性可能反映了其更大的触发BMP 2/Smad 1级联反应的潜力。因此，在“Ta”前面。在骨质疏松症条件下，Ta似乎比Ti更适合作为骨植入物表面材料。
OBJECTIVE: Although Tantalum (Ta) exhibits better osteoinductivity in healthy subjects when compared with titanium (Ti), the relative effects in osteoporosis remain unknown.MATERIALS AND METHODS: In this study, bone mesenchymal stromal cells of ovariectomized rats (OVX-rBMSCs) were seeded on Ta and Ti substrates for in vitro evaluation of cell viability, reactive oxygen species (ROS) production, alkaline phosphatase (ALP) activity, extracellular mineralization osteogenic gene and protein expression involved in bone morphogenetic protein (BMP2)/small mothers against decapentaplegic homologs 1 (Smad1) pathway. For in vivo assessment, Ta and Ti implants were embedded in femur defects of ovariectomized rats, followed by sequential fluorochrome labeling and histological staining.RESULTS: Compared to Ti, the Ta substrates demonstrated higher viable cell percentages (96.5 +/- t 0.26 vs. 88.17 +/- 2.23%), lower ROS levels (65% vs. Ti), and enhanced ALP activity and extracellular matrix calcification. Reverse Transcription-Polymerase Chain Reaction and Western blot assays validated the better osteoinductive effect of Ta regarding small mothers against decapentaplegic homologs 1 (Smad1), runt-related transcription factor 2, bone morphogenetic protein (BMP2), and ALP expression at both the mRNA (1.5-2-fold) and protein (1.2-1.8-fold) levels. BMP2/Smad1 signaling over-expression or knockdown yielded significantly enhanced or deteriorated OVX-rBMSC osteogenesis on the two surfaces. In addition, the Ta group revealed more new bone formation (1.3-1.5-fold vs. Ti) and slightly better bone-implant contact (31.82 +/- 4.07 vs. 25.2-3.84% at 8 weeks post-implantation, p = 0.052) without the contribution of specific surface structures.CONCLUSIONS: In comparison to T1, Ta reveals better biocompatibility and osteoinductivity to OVX-rBMSCs, and the preferential Ta osteoinductivity may reflect its greater potential to trigger the BMP2/Smad1 cascade. Thus," in front of "Ta". Ta appears preferable to Ti as a bone-implant surface material under osteoporosis conditions.