DNA METHYLATION OF EMBRYOGENIC CARROT CELL-CULTURES AND ITS VARIATIONS AS CAUSED BY MUTATION, DIFFERENTIATION, HORMONES AND HYPOMETHYLATING DRUGS
DNA METHYLATION OF EMBRYOGENIC CARROT CELL-CULTURES AND ITS VARIATIONS AS CAUSED BY MUTATION, DIFFERENTIATION, HORMONES AND HYPOMETHYLATING DRUGS
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DOI：
10.1007/bf00305823
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发表时间：
1989-01-01
影响因子：
5.4
通讯作者：
TERZI, M
中科院分区：
文献类型：
--
作者：
LOSCHIAVO, F;PITTO, L;TERZI, M
培养基中天然和合成的生长素水平对培养的胡萝卜细胞 DNA 中 5-甲基胞嘧啶的水平有很大影响。该水平可能在总胞嘧啶的约 15% 至 70% 之间变化，对生长速率和细胞形态没有明显影响。细胞分裂素未见效果。在体细胞胚胎发生过程中，在缺乏激素的情况下，甲基化水平根据特征模式发生变化。如果用氮胞苷、乙硫氨酸或乙氧基羰基嘧啶等药物诱导低甲基化，胚胎发生会立即被阻断。分离出一种对低甲基化药物具有抵抗力的突变体。它显示甲基化模式的变化和吲哚-乙酸代谢的变化。此外，其再生通常与肿瘤的产生有关。
The level of auxin-both natural and synthetic-in the medium has a strong effect on the level of 5-methyl-cytosine in the DNA of carrot cells in culture. This level may vary from approximately 15% to 70% of total cytosine without apparent effects on growth rate and cell morphology. No effect was seen with cytokinin. During somatic embryogenesis, in the absence of hormones, variations were seen in the level of methylation according to a characteristic pattern. If hypomethylation is induced with drugs such as azacytidine, ethionine or ethoxy-carbonyl-pyrimidine, embryogenesis is immediately blocked. A mutant was isolated which is resistant to the action of hypomethylating drugs. It shows variations in the methylation pattern and variations in indole-acetic acid metabolism. In addition its regeneration is often associated with the production of tumors.