Effect of pulse rate on collagen deposition in the tissue-engineered blood vessel
Effect of pulse rate on collagen deposition in the tissue-engineered blood vessel
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DOI：
10.1089/107632703768247287
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发表时间：
2003-08-01
影响因子：
--
通讯作者：
Niklason, L
中科院分区：
文献类型：
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作者：
Solan, A;Mitchell, S;Niklason, L
研究了机械刺激对组织工程血管发育的影响。特别是，选择三种不同的径向扩张速率来产生非脉冲环境（0次/分钟[bpm]）、成人心率（90 bpm）和胎儿心率（165 bpm）。工程化血管平均培养7周。然后分析血管壁的胶原蛋白含量和分布。此外，通过测量活性基质金属蛋白酶1（MMP-1）和金属蛋白酶组织抑制剂1（TIMP-1）水平来评估细胞外基质重塑。在165 bpm扩张速率下生长的血管的胶原蛋白水平显著高于静态条件下生长的血管。MMP-1和TIMP-1水平也较高，脉冲条件下相比，非脉冲条件。对于90-和165-bpm条件，胶原蛋白和MMP-1水平没有显著差异。TIMP-1水平在165 bpm时显著升高，表明对机械刺激的细胞反应增加。机械力及其转导代表了一种可能通过影响细胞外基质沉积和重塑的速率来增强人造血管的物理性质的手段。
The effect of mechanical stimulation on the development of tissue-engineered blood vessels was examined. In particular, three different rates of radial distension were chosen to produce a nonpulsed environment (0 beats per minute [bpm]), an adult heart rate (90 bpm), and a fetal heart rate (165 bpm). Engineered vessels were cultured for an average of 7 weeks. Vessel walls were then analyzed for collagen content and distribution. In addition, extracellular matrix remodeling was assessed through measurement of active matrix metalloproteinase type 1 (MMP-1) and tissue inhibitor of metalloproteinases type 1 (TIMP-1) levels. Vessels grown at a distension rate of 165 bpm had significantly higher collagen levels than those grown under static conditions. MMP-1 and TIMP-1 levels were also higher under pulsed conditions as compared with nonpulsed conditions. For the 90- and 165-bpm conditions, collagen and MMP-1 levels were not significantly different. TIMP-1 levels were significantly elevated at 165 bpm, indicating an increased cellular response to mechanical stimulation. Mechanical forces and their transduction represent a means to enhance the physical properties of artificial blood vessels, possibly by affecting the rate of extracellular matrix deposition and remodeling.