Expression profiling of GH, GHR, and IGF-1 genes in sex-linked dwarf chickens
Expression profiling of GH, GHR, and IGF-1 genes in sex-linked dwarf chickens
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DOI：
10.1360/yc-007-0989
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发表时间：
2007-08-01
期刊：
影响因子：
--
通讯作者：
Yang Ning
中科院分区：
文献类型：
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作者：
Wu Gui-Qin;Zheng Jiang-Xia;Yang Ning
本研究采用实时荧光定量PCR技术比较了伴性矮小鸡及其正常同胞鸡肝脏GH、GHR和IGF-1基因的mRNA表达。结果表明，矮小鸡与正常鸡生长激素的表达无显著差异。矮小鸡GHR基因的表达量是正常鸡的3倍以上。而伴性矮小症患者IGF-1的转录水平显著降低。这些观察结果表明，侏儒表型发生独立的生长激素的作用，但由于功能失调的生长激素受体。据报道，矮小鸡GHR基因第10外显子和3'非翻译区（UTR）有1.7kb的缺失。该转录本可以翻译成不保留其正常功能的蛋白质，这可以解释侏儒鸡肝膜中缺乏GH结合活性的原因。
In this study, quantitative real-time PCR was used to compare the mRNA expression of GH, GHR, and IGF-1 genes in livers of sex-linked dwarf chickens with their normal siblings. The results showed that there was no significant difference between dwarf and normal chickens in GH expression. The expression of GHR gene was more than 3-fold higher in dwarf chickens, compared with that of the normal. However, the transcription level of IGF-1 decreased dramatically in the sex-linked dwarf. These observations suggest that the dwarf phenotype occurs independent of GH action, but is due to a dysfunctional GHR. A 1.7 kb deletion has been reported in exon 10 and the 3' untranslated region (UTR) of the GHR gene in dwarf chickens. This transcript could be translated into a protein that does not retain its normal function, which would account for the absence of GH-binding activity in liver membranes of the dwarf chickens.