Acidic fibroblast growth factor is present in nonneural tissue: isolation and chemical characterization from bovine kidney.
Acidic fibroblast growth factor is present in nonneural tissue: isolation and chemical characterization from bovine kidney.
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酸性成纤维细胞生长因子存在于非神经组织中：从牛肾中分离和化学表征。
DOI：
10.1021/bi00392a039
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
P. Böhlen
中科院分区：
文献类型：
--
作者：
P. Gautschi;Z. Jiang;M. Fràter;P. Böhlen
通过肝素-琼脂糖亲和层析从牛肾纯化的内皮细胞生长因子活性先前被鉴定为碱性成纤维细胞生长因子[Baird，A.，埃施，F.，Böhlen，P.，凌，N.，& Gospodarowicz，D.（1985）Regul. Pept. 12，202-213]。我们现在表明，一个主要的促有丝分裂的馏分，分离肝素琼脂糖纯化材料的单-S阳离子交换色谱和反相高效液相色谱，是有关的酸性成纤维细胞生长因子（aFGF）。序列分析表明，其氨基端序列为Tyr-Lys-Lys-Pro-Lys-Leu-Leu-Tyr-X-Ser-Asn-Gly-Gly-Tyr-Phe-Leu-Arg-Ile-Leu-Pro- Asp-Gly-Thr-Val-Asp-。通过聚丙烯酰胺凝胶电泳测定的蛋白质的分子量为15.5 kDa。结合起来，这些数据强烈表明，这种有丝分裂原是氨基末端截短的酸性成纤维细胞生长因子。到目前为止，aFGF仅在神经组织中发现，即，在大脑和视网膜中。我们的研究结果有力地表明，这种有丝分裂原也发生在神经组织。
Endothelial cell growth factor activity purified from bovine kidney by heparin-Sepharose affinity chromatography was previously identified as basic fibroblast growth factor [Baird, A., Esch, F., Böhlen, P., Ling, N., & Gospodarowicz, D. (1985) Regul. Pept. 12, 202-213]. We now show that a major mitogenic fraction, isolated from heparin-Sepharose-purified material by Mono-S cation-exchange chromatography and reverse-phase high-performance liquid chromatography, is related to acidic fibroblast growth factor (aFGF). Sequence analysis showed the amino-terminal sequence to be Tyr-Lys-Lys-Pro-Lys-Leu-Leu-Tyr-X-Ser-Asn-Gly-Gly-Tyr-Phe-Leu-Arg-Ile-Le u-Pro- Asp-Gly-Thr-Val-Asp-. The molecular mass of the protein, as determined by polyacrylamide gel electrophoresis, was 15.5 kDa. In combination, those data strongly suggest that this mitogen is amino terminally truncated acidic fibroblast growth factor. So far, aFGF has only been found in neural tissues, i.e., in the brain and retina. Our results strongly suggest that this mitogen also occurs in extraneural tissue.
成纤维细胞生长因子的分子特征：在各种组织中的分布和生物活性。
DOI：
10.1016/b978-0-12-571142-5.50008-2
发表时间：
1986
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Baird,A;Esch,F;Mormède,P;Ueno,N;Ling,N;Böhlen,P;Ying,SY;Wehrenberg,WB;Guillemin,R
通讯作者：
Guillemin,R
多种形式的内皮细胞生长因子。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Burgess,WH;Mehlman,T;Friesel,R;Johnson,WV;Maciag,T
通讯作者：
Maciag,T