Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell
Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell
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性腺体细胞异常分化导致生殖细胞减数分裂启动异常
DOI：
10.1155/2019/8030697
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发表时间：
2019-09-05
影响因子：
--
通讯作者：
Gao, Fei
中科院分区：
文献类型：
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作者：
Chen, Min;Chen, Suren;Gao, Fei
生殖细胞和体细胞之间的相互作用在生殖细胞发育中起着重要作用。然而，性腺体细胞在生殖细胞分化中的确切功能尚不清楚。本研究利用体细胞分化异常的小鼠模型，探讨了性腺体细胞在生殖细胞减数分裂中的作用。在Wt 1（R394 W/R394 W）小鼠中，由于体腔上皮细胞的凋亡，生殖嵴缺失。有趣的是，在雄性和雌性Wt 1（R394 W/R394 W）生殖细胞中，在E12.5时检测到STRA 8，在E13.5时观察到分散的SYCP 3病灶，这与对照雌性一致。int findDuplicate（）; Cre-ERTM小鼠，在E9.5注射他莫昔芬使Wt 1失活，支持细胞和颗粒细胞的分化被完全阻断。我们发现，大多数生殖细胞位于外生殖嵴Wt 1失活后。在雄性和雌性Wt 1（-/flox）; Cre-ERTM性腺的生殖细胞中均检测到STRA 8、SYCP 3和γ H2 AX蛋白，而未观察到线状SYCP 3信号。我们的研究表明，性腺体细胞的异常发育导致减数分裂相关基因在生殖细胞中的异位表达，但减数分裂在前期I之前被阻止。这些结果表明，性腺体细胞的适当分化是生殖细胞减数分裂所必需的。
The interaction between germ cell and somatic cell plays important roles in germ cell development. However, the exact function of gonad somatic cell in germ cell differentiation is unclear. In the present study, the function of gonad somatic cell in germ cell meiosis was examined by using mouse models with aberrant somatic cell differentiation. In Wt1(R394W/R394W) mice, the genital ridge is absent due to the apoptosis of coelomic epithelial cells. Interestingly, in both male and female Wt1(R394W/R394W) germ cells, STRA8 was detected at E12.5 and the scattered SYCP3 foci were observed at E13.5 which was consistent with control females. In Wt1(-/flox); Cre-ERTM mice, Wt1 was inactivated by the injection of tamoxifen at E9.5 and the differentiation of Sertoli and granulosa cells was completely blocked. We found that most germ cells were located outside of genital ridge after Wt1 inactivation. STRA8, SYCP3, and gamma H2AX proteins were detected in germ cells of both male and female Wt1(-/flox); Cre-ERTM gonads, whereas no thread-like SYCP3 signal was observed. Our study demonstrates that aberrant development of gonad somatic cells leads to ectopic expression of meiosis-associated genes in germ cells, but meiosis was arrested before prophase I. These results suggest that the proper differentiation of gonad somatic cells is essential for germ cell meiosis.