Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames
Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames
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DOI：
10.1099/vir.0.81727-0
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发表时间：
2006-06-01
影响因子：
3.8
通讯作者：
Maruniak, James E.
中科院分区：
文献类型：
--
作者：
Lauzon, Hilary A. M.;Garcia-Maruniak, Alejandra;Maruniak, James E.
膜翅目杆状病毒NeseNPV和NeleNPV的基因组比较表明，膜翅目杆状病毒既有共同的特征，又有别于其他全序列的鳞翅目和双翅目杆状病毒。与其他杆状病毒相比，它们的基因组较小(分别为86 462和81 755个碱基)，G+C含量较低(分别为33.8和33.3%)，开放阅读框(ORF)较少(分别为90和89)。它们共有69个ORF(48.6%的氨基酸平均同源性)，其中43个是以前鉴定的杆状病毒同源物。其余共有的开放阅读框可能是其他杆状病毒共有的，但低氨基酸的同一性排除了将它们识别为杆状病毒的可能性。有些也可能是膜翅目杆状病毒所特有的。这些蛋白包括类胰蛋白酶、锌指蛋白、染色体凝聚蛋白的调节因子、致密病毒衣壳蛋白和磷酸转移酶。结构分析、保守结构域的存在和系统发育研究表明，其中一些ORF可能具有功能，可能是从昆虫宿主水平转移过来的。仅在NeseNPV和NeleNPV中发现的ORF可能在寄主特异性和/或组织嗜性中发挥作用，因为膜翅目杆状病毒仅限于中肠。基因组基本共线，但含有非共线区，多角体基因和DBP基因之间有大量重复。它们在ORF的数量、NSRS的G+C含量以及NeseNPV基因组中同源区域的存在方面存在差异。与NeseNPV相比，NeleNPV也有一个短暂的倒置。NeseNPV含有21个在NeleNPV中没有的ORF，NeleNPV有20个在NeseNPV中没有发现的ORF。
Genomic comparison of Neodiprion sertifer nucleopolyhedrovirus (NeseNPV) and Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) showed that the hymenopteran baculoviruses had features in common and were distinct from other, fully sequenced lepidopteran and dipteran baculoviruses. Their genomes were small in size (86 462 and 81 755 bp, respectively), had low G+C contents (33.8 and 33.3 mol%, respectively) and contained fewer open reading frames (ORFs) (90 and 89, respectively) than other baculoviruses. They shared 69 ORFs (48.6% mean amino acid identity overall), 43 of which were previously identified baculovirus homologues. The remaining shared ORFs could be common to other baculoviruses, but low amino acid identities precluded identifying them as such. Some may also be unique to hymenopteran baculoviruses. These included a trypsin-like protease, a zinc-finger protein, regulator of chromosome condensation proteins, a densovirus capsid-like protein and a phosphotransferase. Structural analysis, the presence of conserved domains and phylogenetic studies suggested that some of these ORFs may be functional and could have been transferred horizontally from an insect host. ORFs found only in NeseNPV and NeleNPV may play a role in host specificity and/or tissue tropism, as hymenopteran baculoviruses are restricted to the midgut. The genomes were basically collinear, but contained non-syntenic regions (NSRs) with large numbers of repeats between their polyhedrin and dbp genes. They differed from each other in the number of ORFs and the G+C content of their NSRs and the presence of homologous regions in the NeseNPV genome. NeleNPV also had a short inversion relative to NeseNPV. NeseNPV contained 21 ORFs not found in NeleNPV and NeleNPV had 20 ORFs not found in NeseNPV.