Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge
Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge
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DOI：
10.1016/s0142-9612(98)00180-x
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发表时间：
1999-03-01
期刊：
影响因子：
14
通讯作者：
Nam, YS
中科院分区：
文献类型：
--
作者：
Choi, YS;Hong, SR;Nam, YS
以明胶和海藻酸钠为原料，采用新的交联方法制备了可吸收海绵，提高了交联效率。交联度通过三硝基苯磺酸（TNBS）测定来表征。采用吸水能力测试、体外药物释放和胶原酶降解测试以及体内动物试验来确认这种明胶-藻酸盐海绵作为伤口敷料材料的适用性。随着海绵中海藻酸盐含量的增加，孔隙率增加，导致吸水能力增强。装有磺胺嘧啶银或硫酸庆大霉素的海绵缓慢释放药物长达四天。交联海绵抵抗体外胶原酶消化长达三天。用Wistar大鼠进行的动物实验表明，在我们的全层皮肤缺损模型中，含AgSD的明胶-藻酸盐海绵比凡士林纱布具有更好的伤口愈合效果。(C)1999 Elsevier Science Ltd.保留所有权利。
An absorbable sponge, composed of gelatin and alginate, was prepared by new crosslinking method that improved the efficiency of crosslinking. The crosslinking degree was characterized by trinitrobenzenesulfonic acid (TNBS) assay. A water uptake ability test, in vitro drug release and collagenase degradation tests, and an in vivo animal test were employed to confirm the applicability of this gelatin-alginate sponge as a wound dressing material. As the alginate content in the sponge increased, the porosity increased, resulting in an enhanced water uptake ability. Sponges loaded with silver sulfadiazine or gentamicin sulfate slowly released drugs for up to four days. The crosslinked sponge resisted in vitro collagenase digestion for up to three days. An in vivo animal test using witar rat showed rather good wound healing effect of gelatin-alginate sponge containing AgSD than vaseline gauze in our full-thickness skin defect model. (C) 1999 Elsevier Science Ltd. All rights reserved.